Cloned (Comment) | Organism |
---|---|
gene pgmA, sequence comparison, phylogenetic analysis and expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-diphosphate | noncompetitive inhibitor | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Mycobacterium tuberculosis | |
0.1 | 2 | alpha-D-glucose 1-phosphate | recombinant enzyme, pH 7.4, 30°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | Mycobacterium tuberculosis | - |
D-glucose 6-phosphate | - |
r | |
alpha-D-glucose 1-phosphate | Mycobacterium tuberculosis H37Rv | - |
D-glucose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | I6Y2G3 | gene pgmA | - |
Mycobacterium tuberculosis H37Rv | I6Y2G3 | gene pgmA | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, protein refolding, and ultrafiltration | Mycobacterium tuberculosis |
Renatured (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) inclusion bodies | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67.5 | - |
purified recombinant enzyme, pH 7.4, 30°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis | D-glucose 6-phosphate | - |
r | |
alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis H37Rv | D-glucose 6-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | four-domain architecture, homology modeling, overview | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
alpha phosphoglucomutase | - |
Mycobacterium tuberculosis |
PGM | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | recombinant enzyme | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70.31 | - |
alpha-D-glucose 1-phosphate | recombinant enzyme, pH 7.4, 30°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mycobacterium tuberculosis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.4 | purified recombinant enzyme, stable at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
additional information | structure homology modeling, overview | Mycobacterium tuberculosis |
physiological function | the enzyme plays an important role in polysaccharide capsule formation and virulence in a number of bacterial pathogens | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
58.4 | - |
alpha-D-glucose 1-phosphate | recombinant enzyme, pH 7.4, 30°C | Mycobacterium tuberculosis |