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Literature summary for 5.4.2.2 extracted from
- Characterization of alpha-phosphoglucomutase isozymes from Toxoplasma gondii (2010), Parasitol. Int., 59, 206-210.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-Glucose 1,6-bisphosphate | the maximum activity is obtained in the presence of more than 0.2 mM alpha-D-glucose 1,6-bisphosphate | Toxoplasma gondii |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Toxoplasma gondii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
alpha-D-glucose 1-phosphate | isozyme PGM-I, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii | |
| 3.53 | - |
alpha-D-glucose 1-phosphate | isozyme PGM-II, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ are the most effective divalent cations for the enzyme activity of isozyme PGM-I | Toxoplasma gondii | |
| Mg2+ | Mg2+ are the most effective divalent cations for the enzyme activity of isozyme PGM-II | Toxoplasma gondii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Toxoplasma gondii | C6L2E2 | isozyme PGM-I | - |
| Toxoplasma gondii | C6L2F4 | isozyme PGM-II | - |
| Toxoplasma gondii Beverley | C6L2E2 | isozyme PGM-I | - |
| Toxoplasma gondii Beverley | C6L2F4 | isozyme PGM-II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione Sepharose column chromatography | Toxoplasma gondii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tachyzoite | - |
Toxoplasma gondii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 84 | - |
isozyme PGM-II, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii |
| 338 | - |
isozyme PGM-I, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | - |
Toxoplasma gondii | alpha-D-glucose 6-phosphate | - |
? | |
| alpha-D-glucose 1-phosphate | - |
Toxoplasma gondii Beverley | alpha-D-glucose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-phosphoglucomutase | - |
Toxoplasma gondii |
| PGM-I | isozyme | Toxoplasma gondii |
| PGM-II | isozyme | Toxoplasma gondii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 93 | - |
alpha-D-glucose 1-phosphate | isozyme PGM-II, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii | |
| 398 | - |
alpha-D-glucose 1-phosphate | isozyme PGM-I, in 50 mM HEPES-KOH, pH 8.0, at 37°C | Toxoplasma gondii | |
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