Literature summary for 5.4.2.2 extracted from

Neves, A.R.; Pool, W.A.; Castro, R.; Mingote, A.; Santos, F.; Kok, J.; Kuipers, O.P.; Santos, H.
The alpha-phosphoglucomutase of Lactococcus lactis is unrelated to the alpha-D-phosphohexomutase superfamily and is encoded by the essential gene pgmH (2006), J. Biol. Chem., 281, 36864-36873.

Search for more literature for 5.4.2.2

Activating Compound

Activating Compound	Comment	Organism	Structure
glucose 1,6-bisphosphate	absolutely required	Lactococcus lactis

Cloned(Commentary)

Cloned (Comment)	Organism
-	Lactococcus lactis

Protein Variants

Protein Variants	Comment	Organism
additional information	overproduction of enzyme leads to increased glycolytic flux and growth rate on galactose. Growth of a conditional knock-out strain is severely impaired when enzyme activity is below the control level	Lactococcus lactis

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	-	Lactococcus lactis
D-fructose 1,6-bisphosphate	-	Lactococcus lactis
Zn2+	-	Lactococcus lactis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0168	-	alpha-D-glucose 1-phosphate	pH 7.2, 30°C	Lactococcus lactis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required, less than 2% of activity in absence of Mg2+	Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	3 * 28276, calculated, 3 * 28000, SDS-PAGE	Lactococcus lactis
28276	-	3 * 28276, calculated, 3 * 28000, SDS-PAGE	Lactococcus lactis
84300	-	gel filtration	Lactococcus lactis

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis	Q00G41	-	-

Purification (Commentary)

Purification (Comment)	Organism
both native and recombinant enzyme	Lactococcus lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucose 1-phosphate	enzyme is highly specific for substrate and product	Lactococcus lactis	alpha-D-glucose 6-phosphate	-	r
alpha-D-glucose 6-phosphate	23% of the activity with alpha-D-glucose 1-phosphate	Lactococcus lactis	alpha-D-glucose 1-phosphate	-	r
additional information	no substrate: alpha-D-mannose 1-phosphate, beta-D-glucose 1-phosphate	Lactococcus lactis	?	-	?

Subunits

Subunits	Comment	Organism
trimer	3 * 28276, calculated, 3 * 28000, SDS-PAGE	Lactococcus lactis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.6	7.5	more than 50% of maximum activity within this range	Lactococcus lactis
6.5	-	-	Lactococcus lactis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
48	-	D-fructose 1,6-bisphosphate	pH 7.2, 30°C	Lactococcus lactis

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Release 2023.1 (January 2023)