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Literature summary for 5.4.2.12 extracted from

  • Singh, M.; Jamal, F.; Dubey, A.; Shivam, P.; Kumari, S.; Pushpanjal, P.; Ahmed, G.; Dikhit, M.; Narayan, S.; Das, V.; Pandey, K.; Sinha, K.; Das, P.; Singh, S.
    Co-factor-independent phosphoglycerate mutase of Leishmania donovani modulates macrophage signalling and promotes T-cell repertoires bearing epitopes for both MHC-I and MHC-II (2018), Parasitology, 145, 292-306 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR(DE3) cells Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Leishmania donovani 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Leishmania donovani
-
3-phospho-D-glycerate
-
r
3-phospho-D-glycerate Leishmania donovani
-
2-phospho-D-glycerate
-
r

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA bead chromatography Leishmania donovani

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Leishmania donovani 3-phospho-D-glycerate
-
r
3-phospho-D-glycerate
-
Leishmania donovani 2-phospho-D-glycerate
-
r

Subunits

Subunits Comment Organism
monomer 1 * 61000, SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
co-factor-independent phosphoglycerate mutase
-
Leishmania donovani
iPGAM
-
Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme is independent from 2,3-bisphosphoglycerate Leishmania donovani

General Information

General Information Comment Organism
physiological function the enzyme modulates macrophage signaling and promotes T-cell repertoires bearing epitopes for both major histocompatibility complex classes I and II. Enzyme stimulation induces higher expression of interleukin (IL)-1beta in the phagocytic cell, its receptor and CD69 on T-cell subsets. These cellular activations result in upregulation of host-protective cytokines IL-2, IL-12, IL-17, tumour necrosis factor-alpha and interferon-gamma and downregulation of IL-4, IL-10 and tumour growth factor-beta. Enzyme stimulation also promotes lymphocyte proliferation and boosted the leishmaniacidal activity of macrophages by upregulating reactive oxygen species. It also induces 1.8fold higher release of nitric oxide by promoting the transcription of inducible nitric oxide synthase gene Leishmania donovani