Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BLR(DE3) cells | Leishmania donovani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania donovani | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Leishmania donovani | - |
3-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | Leishmania donovani | - |
2-phospho-D-glycerate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA bead chromatography | Leishmania donovani |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Leishmania donovani | 3-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | - |
Leishmania donovani | 2-phospho-D-glycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 61000, SDS-PAGE | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
co-factor-independent phosphoglycerate mutase | - |
Leishmania donovani |
iPGAM | - |
Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is independent from 2,3-bisphosphoglycerate | Leishmania donovani |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme modulates macrophage signaling and promotes T-cell repertoires bearing epitopes for both major histocompatibility complex classes I and II. Enzyme stimulation induces higher expression of interleukin (IL)-1beta in the phagocytic cell, its receptor and CD69 on T-cell subsets. These cellular activations result in upregulation of host-protective cytokines IL-2, IL-12, IL-17, tumour necrosis factor-alpha and interferon-gamma and downregulation of IL-4, IL-10 and tumour growth factor-beta. Enzyme stimulation also promotes lymphocyte proliferation and boosted the leishmaniacidal activity of macrophages by upregulating reactive oxygen species. It also induces 1.8fold higher release of nitric oxide by promoting the transcription of inducible nitric oxide synthase gene | Leishmania donovani |