Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 (pREP4) cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using either 0.1 M HEPES pH 7.5, 20% (w/v) polyethylene glycol 10000 or 0.2 M NaCl, 0.1 M bis-Tris pH 6.5, 25% (w/v) polyethylene glycol 3350, at 25°C | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | dependent on | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Staphylococcus aureus | - |
3-phospho-D-glycerate | - |
? | |
2-phospho-D-glycerate | Staphylococcus aureus NCTC 8325 | - |
3-phospho-D-glycerate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q2G029 | - |
- |
Staphylococcus aureus NCTC 8325 | Q2G029 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sepharose column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Staphylococcus aureus | 3-phospho-D-glycerate | - |
? | |
2-phospho-D-glycerate | - |
Staphylococcus aureus NCTC 8325 | 3-phospho-D-glycerate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
iPGM | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not dependent from 2,3-bisphosphoglycerate | Staphylococcus aureus |