Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using either 40% (v/v) ethanol, 5% (w/v) PEG 1000, and 0.1 M phosphate citrate pH 4.2, or 20% (w/v) PEG 3350, 0.2 M magnesium acetate and pH 8.5 | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucosamine 1-phosphate | Aquifex aeolicus | - |
alpha-D-glucosamine 6-phosphate | - |
? | |
alpha-D-glucosamine 1-phosphate | Aquifex aeolicus VFS | - |
alpha-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67549 | - |
- |
Aquifex aeolicus VFS | O67549 | - |
- |
Purification (Comment) | Organism |
---|---|
SuperQ Toyopearl 650 column chromatography, ResourceQ column chromatography, and Superdex 200 gel filtration | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucosamine 1-phosphate | - |
Aquifex aeolicus | alpha-D-glucosamine 6-phosphate | - |
? | |
alpha-D-glucosamine 1-phosphate | - |
Aquifex aeolicus VFS | alpha-D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 13000, calculated from amino acid sequence | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
alpha-phosphoglucoseamine mutase | - |
Aquifex aeolicus |
alpha-PNGM | - |
Aquifex aeolicus |
Aq1627 | - |
Aquifex aeolicus |
GlmM | - |
Aquifex aeolicus |
PNGM | - |
Aquifex aeolicus |