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Literature summary for 5.4.2.10 extracted from

  • Sridharan, U.; Kuramitsu, S.; Yokoyama, S.; Kumarevel, T.; Ponnuraj, K.
    Crystal structure of Aquifex aeolicus gene product Aq1627 a putative phosphoglucosamine mutase reveals a unique C-terminal end-to-end disulfide linkage (2017), Mol. Biosyst., 13, 1370-1376 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using either 40% (v/v) ethanol, 5% (w/v) PEG 1000, and 0.1 M phosphate citrate pH 4.2, or 20% (w/v) PEG 3350, 0.2 M magnesium acetate and pH 8.5 Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucosamine 1-phosphate Aquifex aeolicus
-
alpha-D-glucosamine 6-phosphate
-
?
alpha-D-glucosamine 1-phosphate Aquifex aeolicus VFS
-
alpha-D-glucosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O67549
-
-
Aquifex aeolicus VFS O67549
-
-

Purification (Commentary)

Purification (Comment) Organism
SuperQ Toyopearl 650 column chromatography, ResourceQ column chromatography, and Superdex 200 gel filtration Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucosamine 1-phosphate
-
Aquifex aeolicus alpha-D-glucosamine 6-phosphate
-
?
alpha-D-glucosamine 1-phosphate
-
Aquifex aeolicus VFS alpha-D-glucosamine 6-phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 13000, calculated from amino acid sequence Aquifex aeolicus

Synonyms

Synonyms Comment Organism
alpha-phosphoglucoseamine mutase
-
Aquifex aeolicus
alpha-PNGM
-
Aquifex aeolicus
Aq1627
-
Aquifex aeolicus
GlmM
-
Aquifex aeolicus
PNGM
-
Aquifex aeolicus