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Literature summary for 5.4.2.10 extracted from
- Acetamido sugar biosynthesis in the Euryarchaea (2008), J. Bacteriol., 190, 2987-2996.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose-1,6-bisphosphate | required to activate the enzyme | Methanococcus maripaludis |  |
| dithiothreitol | 1 mM, required for maximal activity | Methanococcus maripaludis | |
| additional information | no activity is observed with ATP as an activator | Methanococcus maripaludis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Methanococcus maripaludis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity is 95% lower when MgCl2 is used instead of MnCl2 | Methanococcus maripaludis | |
| Mn2+ | required for activity | Methanococcus maripaludis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Methanococcus maripaludis |
| 340000 | - |
gel filtration | Methanococcus maripaludis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanococcus maripaludis | Q6LYB4 | strain 900 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni+ affinity column chromatography | Methanococcus maripaludis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucosamine 6-phosphate | - |
Methanococcus maripaludis | alpha-D-glucosamine 1-phosphate | - |
r | |
| alpha-D-glucose 1-phosphate | - |
Methanococcus maripaludis | alpha-D-glucose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlmM | - |
Methanococcus maripaludis |
| MMP1077 | - |
Methanococcus maripaludis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
alpha-D-glucose 1-phosphate | - |
Methanococcus maripaludis | |
| 0.52 | - |
alpha-D-glucosamine 1-phosphate | - |
Methanococcus maripaludis | |
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Release 2023.1 (January 2023)
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