Literature summary for 5.4.2.10 extracted from

Jolly, L.; Pompeo, F.; van Heijenoort, J.; Fassy, F.; Mengin-Lecreulx, D.
Autophosphorylation of phosphoglucosamine mutase from Escherichia coli (2000), J. Bacteriol., 182, 1280-1285.

Search for more literature for 5.4.2.10

Activating Compound

Activating Compound	Comment	Organism	Structure
D-glucosamine 1,6-diphosphate	retains the enzyme in an active and phosphorylated form	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
S100A	50fold less active	Escherichia coli
S102A	completely inactive, loss of autophosphorylation ability	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	80% reduced activity	Escherichia coli
EDTA	abolishes autophosphorylation and catalytic activity	Escherichia coli
Mn2+	80% reduced activity	Escherichia coli
Zn2+	efficient autophosphorylation, but absence of catalytic activity	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential for autophosphorylation and catalytic activity	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucoamine 6-phosphate	Escherichia coli	ping-pong bi-bi reaction mechanism	D-glucosamine 1-phosphate	-	r
D-glucoamine 6-phosphate	Escherichia coli	involved in formation of cell-wall precursor UDP-N-acetylglucosamine	D-glucosamine 1-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	in vitro autophosphorylation at S102 in the presence of [gamma-32P]ATP	Escherichia coli

Purification (Commentary)

Purification (Comment)	Organism
near homogeneity, recombinant enzyme	Escherichia coli

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Escherichia coli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucoamine 6-phosphate	ping-pong bi-bi reaction mechanism	Escherichia coli	D-glucosamine 1-phosphate	-	r
D-glucoamine 6-phosphate	involved in formation of cell-wall precursor UDP-N-acetylglucosamine	Escherichia coli	D-glucosamine 1-phosphate	-	r

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Release 2023.1 (January 2023)