Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TOPO10 cells | Capsicum annuum |
Protein Variants | Comment | Organism |
---|---|---|
E295A | the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS | Capsicum annuum |
E295K | the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS | Capsicum annuum |
E295R | the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS | Capsicum annuum |
H360A | the mutant shows completely abolished activity | Capsicum annuum |
H360K | the mutant shows partially recovered activity | Capsicum annuum |
H360R | the mutant shows partially recovered activity | Capsicum annuum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromoplast | - |
Capsicum annuum | 9509 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
1 * 66000, SDS-PAGE | Capsicum annuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | Q42435 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Affigel 501 gel filtration, Mono-P column chromatography, and Sephacryl gel filtration, and Superose 6 gel filtration | Capsicum annuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
antheraxanthin | - |
Capsicum annuum | capsanthin + 5,6-diepikarpoxanthin | - |
? | |
additional information | CCS is mainly dedicated to the synthesis of kappa-cyclic carotenoids (capsanthin and capsorubin) but also has lycopene beta-cyclase activity | Capsicum annuum | ? | - |
? | |
violaxanthin | - |
Capsicum annuum | capsorubin + capsanthin-5,6-epoxide + 5,6-diepilatoxanthin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Capsicum annuum |
Synonyms | Comment | Organism |
---|---|---|
capsanthin-capsorubin synthase | - |
Capsicum annuum |
CCS | - |
Capsicum annuum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant | Capsicum annuum | |
NADPH | the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant | Capsicum annuum |