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Literature summary for 5.3.99.8 extracted from

  • Mialoundama, A.S.; Heintz, D.; Jadid, N.; Nkeng, P.; Rahier, A.; Deli, J.; Camara, B.; Bouvier, F.
    Characterization of plant carotenoid cyclases as members of the flavoprotein family functioning with no net redox change (2010), Plant Physiol., 153, 970-979.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TOPO10 cells Capsicum annuum

Protein Variants

Protein Variants Comment Organism
E295A the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS Capsicum annuum
E295K the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS Capsicum annuum
E295R the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS Capsicum annuum
H360A the mutant shows completely abolished activity Capsicum annuum
H360K the mutant shows partially recovered activity Capsicum annuum
H360R the mutant shows partially recovered activity Capsicum annuum

Localization

Localization Comment Organism GeneOntology No. Textmining
chromoplast
-
Capsicum annuum 9509
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
1 * 66000, SDS-PAGE Capsicum annuum

Organism

Organism UniProt Comment Textmining
Capsicum annuum Q42435
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Affigel 501 gel filtration, Mono-P column chromatography, and Sephacryl gel filtration, and Superose 6 gel filtration Capsicum annuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
antheraxanthin
-
Capsicum annuum capsanthin + 5,6-diepikarpoxanthin
-
?
additional information CCS is mainly dedicated to the synthesis of kappa-cyclic carotenoids (capsanthin and capsorubin) but also has lycopene beta-cyclase activity Capsicum annuum ?
-
?
violaxanthin
-
Capsicum annuum capsorubin + capsanthin-5,6-epoxide + 5,6-diepilatoxanthin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Capsicum annuum

Synonyms

Synonyms Comment Organism
capsanthin-capsorubin synthase
-
Capsicum annuum
CCS
-
Capsicum annuum

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant Capsicum annuum
NADPH the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant Capsicum annuum