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Literature summary for 5.3.99.7 extracted from

  • Han, J.H.; Park, M.S.; Bae, J.W.; Lee, E.Y.; Yoon, Y.J.; Lee, S.; Park, S.
    Production of (S)-styrene oxide using styrene oxide isomerase negative mutant of Pseudomonas putida SN1 (2006), Enzyme Microb. Technol., 39, 1264-1269.
No PubMed abstract available

Application

Application Comment Organism
synthesis enzyme disruption mutant is unable to grow on styrene or styrene oxide and accumulates enantiopure (S)-styrene when incubated with styrene. Upon growth on citrate as carbon source, mutant strain exhibits a high whole-cell activity of styrene oxide formation. In a 3 l bioreactor experiment, mutant can accumulate 150 mM (S)-styrene oxide at an enantiomerixc excess above 98% in 13 h Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information enzyme disruption mutant is unable to grow on styrene or styrene oxide and accumulates enantiopure (S)-styrene when incubated with styrene. Upon growth on citrate as carbon source, mutant strain exhibits a high whole-cell activity of styrene oxide formation Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
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Pseudomonas putida SN1
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Synonyms

Synonyms Comment Organism
StyC
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Pseudomonas putida