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Literature summary for 5.3.99.6 extracted from

  • Riemann, M.; Haga, K.; Shimizu, T.; Okada, K.; Ando, S.; Mochizuki, S.; Nishizawa, Y.; Yamanouchi, U.; Nick, P.; Yano, M.; Minami, E.; Takano, M.; Yamane, H.; Iino, M.
    Identification of rice allene oxide cyclase mutants and the function of jasmonate for defence against Magnaporthe oryzae (2013), Plant J., 74, 226-238.
    View publication on PubMed
Search for more literature for 5.3.99.6

Cloned(Commentary)

Cloned (Comment) Organism
gene OsAOC, OsAOC lacking the chloroplast transit peptide is amplified from cultivar Nihonmasari cDNA and expressed as GST-tagged enzyme in Escherichia coli strain BL21 Oryza sativa

Protein Variants

Protein Variants Comment Organism
additional information two photomorphogenic mutants of rice, coleoptile photomorphogenesis 2 (cpm2) and hebiba, are defective in the gene encoding allene oxide cyclase (OsAOC), map-based cloning and complementation assays, overview Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate Oryza sativa
-
 (15Z)-12-oxophyto-10,15-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Q8L6H4 cv. Nihonmasari
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged OsAOC lacking the chloroplast transit peptide from Escherichia coli strain BL21 by glutathione affinity chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate
-
 Oryza sativa (15Z)-12-oxophyto-10,15-dienoate
-
 ?

Synonyms

Synonyms Comment Organism
Allene oxide cyclase
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Oryza sativa

General Information

General Information Comment Organism
malfunction two photomorphogenic mutants of rice, coleoptile photomorphogenesis 2 (cpm2) and hebiba, are defective in the gene encoding allene oxide cyclase, phenotypes, overview. Both mutants are more susceptible than wild-type to an incompatible strain of Magnaporthe oryzae in such a way that hyphal growth is enhanced in mutant tissues. Blast-induced accumulation of phytoalexins, especially that of the flavonoid sakuranetin, is severely impaired in cpm2 and hebiba Oryza sativa
metabolism the enzyme is involved in the biosynthesis of jasmonic acid and related compounds Oryza sativa
physiological function the rice allene oxide cyclase functions in defence against blast fungus Magnaporthe oryzae through the pathway of jasmonate production Oryza sativa