Cloned (Comment) | Organism |
---|---|
expressed in 293-F cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H72A | the mutation markedly decreases the mPGES-1 activity (32%) | Homo sapiens |
N74A | the mutant shows increased Km compared to the wild type enzyme | Homo sapiens |
R122A | the mutant shows increased Km compared to the wild type enzyme | Homo sapiens |
R70A | the mutant shows increased Km compared to the wild type enzyme | Homo sapiens |
R70A/R122A | the mutant shows increased Km compared to the wild type enzyme, the double mutant of the mPGES-1 significantly reduces the binding affinity of GSH | Homo sapiens |
T129V | the mutant shows increased Km compared to the wild type enzyme | Homo sapiens |
Y117A | the mutant shows increased Km compared to the wild type enzyme | Homo sapiens |
Y117F | the mutant still maintains full catalytic activity relative to the wild type | Homo sapiens |
Y117S | the mutant shows increased Km compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.215 | - |
prostaglandin H2 | wild type enzyme | Homo sapiens | |
0.2832 | - |
prostaglandin H2 | mutant enzyme Y117S | Homo sapiens | |
0.296 | - |
prostaglandin H2 | mutant enzyme R70A | Homo sapiens | |
0.372 | - |
prostaglandin H2 | mutant enzyme R122A | Homo sapiens | |
0.3842 | - |
prostaglandin H2 | mutant enzyme T129V | Homo sapiens | |
0.4643 | - |
prostaglandin H2 | mutant enzyme N74A | Homo sapiens | |
0.4737 | - |
prostaglandin H2 | mutant enzyme R70A/R122A | Homo sapiens | |
0.5345 | - |
prostaglandin H2 | mutant enzyme Y117A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14684 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin H2 | - |
Homo sapiens | Prostaglandin E2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
microsomal prostaglandin E synthase-1 | - |
Homo sapiens |
mPGES-1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Homo sapiens |