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Literature summary for 5.3.99.3 extracted from

  • Hammarberg, T.; Hamberg, M.; Wetterholm, A.; Hansson, H.; Samuelsson, B.; Haeggstroem, J.Z.
    Mutation of a critical arginine in microsomal prostaglandin E synthase-1 shifts the isomerase activity to a reductase activity that converts prostaglandin H2 into prostaglandin F2{alpha} (2009), J. Biol. Chem., 284, 301-305.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.99.3

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pPICZA for overexpression in Pichia pastoris cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
R126A mutant, exhibits a glutathione-dependent reductase activity, which allows conversion of prostaglandin H2 into prostaglandin F2alpha Homo sapiens
R126Q mutant, exhibits a glutathione-dependent reductase activity, which allows conversion of prostaglandin H2 into prostaglandin F2alpha Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 determined by SDS-PAGE and Western Blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate Homo sapiens PGH2 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate PGE2 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O14684
-
-

Purification (Commentary)

Purification (Comment) Organism
microsomes are prepared Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate PGH2 Homo sapiens (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate PGE2 ?
additional information (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate, PGH2, mutation R126A/Q creates a glutathione-dependent reductase which is able to convert prostaglandin H2 to prostaglandin F2alpha Homo sapiens additional information (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate, PGF2alpha ?

Synonyms

Synonyms Comment Organism
microsomal prostaglandin E synthase type 1
-
 Homo sapiens
microsomal prostaglandin E synthase-1
-
 Homo sapiens
mPGES-1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 microsomal suspension is incubated on ice with PGH2, enzyme activity assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 enzyme activity assay Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information mutants R126A/Q possess glutathione-dependent reductase activity Homo sapiens