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Literature summary for 5.3.99.3 extracted from
- Expression and purification of human mPGES-1 in E. coli and identification of inhibitory compounds from a drug-library (2008), BMB Rep., 41, 808-813.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the selective inhibition of mPGES-1 generates anti-inflammatory effects without unwanted side effects involving homeostasis | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the TA vector and subsequently into pET-28a for expression in various Escherichia coli strains, the expression rate is enhanced by modifying the utility of specific codons | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dyphylline | - |
Homo sapiens |  |
| Oxacillin | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18800 | - |
determined by SDS-PAGE and Western Blot analysis | Homo sapiens |
| 19000 | - |
calculated, His-tagged mPGES-1 | Homo sapiens |
| 57000 | - |
trimer, determined by SDS-PAGE and Western Blot analysis | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | Homo sapiens | - |
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Ni-NTA and a Mono Q-Sepharose column | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Homo sapiens | (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | trimeric state characterized by chemical cross-linking with glutaraldehyde | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| microsomal prostaglandin E synthase-1 | - |
Homo sapiens |
| mPGES-1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
activity assay at room temperature | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
- |
Homo sapiens | Oxacillin | |
| 0.23 | - |
- |
Homo sapiens | dyphylline | |
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