Literature summary for 5.3.99.3 extracted from

Yamada, T.; Takusagawa, F.
PGH2 degradation pathway catalyzed by GSH-heme complex bound microsomal prostaglandin E2 synthase type 2: the first example of a dual-function enzyme (2007), Biochemistry, 46, 8414-8424.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminally truncated enzyme in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
red-coloured protein purified after growth of Escherichia coli in LB medium containing delta-aminolevulinate and Fe(NO3)3. Enzyme contains bound glutathione and heme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isoform mPGES-2	-

Purification (Commentary)

Purification (Comment)	Organism
purification after expression of N-terminally truncated enzyme in recombinant Escherichia coli growing in LB medium containing delta-aminolevulinate and Fe(NO3)3 gives a protein of red colour. Purification after growth of Escherichia coli on minimal medium gives a colourless protein	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	glutathione-heme-complex bound enzyme catalyzes formation of 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid and malonaldehyde from prostaglandin H2, i.e. shows lyase activity	Homo sapiens	?	-	?

Cofactor

Cofactor	Comment	Organism	Structure
heme	crystallization data of red-coloured protein purified after growth of recombinant Escherichia coli in LB medium containing delta-aminolevulinate and Fe(NO3)3. Heme is complexed with bound glutathione forming a S-Fe coordination bond with no polar interaction with enzyme. Heme dissociation constant is 0.00053 mM	Homo sapiens

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Release 2023.1 (January 2023)