Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K401Q | the mutant shows about 1.5fold less activity than the wild type enzyme | Homo sapiens |
K57A/K401A | the mutant shows about 4fold less activity than the wild type enzyme | Homo sapiens |
K57E/K401E | the mutant shows about 7fold less activity than the wild type enzyme | Homo sapiens |
K57Q | the mutant shows about 1.5fold less activity than the wild type enzyme | Homo sapiens |
K57Q/K401Q | the mutant shows about 2.5fold less activity than the wild type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07237 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
acetylation | acetylation of the active site-flanking lysine residues can act to reversibly modulate enzyme activity | Homo sapiens |
S-nitrosylation | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
Ni-affinity resin column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dieosin glutathione disulfide | - |
Homo sapiens | eosin glutathione sulfide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDI | - |
Homo sapiens |
protein disulfide isomerase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Homo sapiens |