Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant PDI in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C294S/C325S | site-directed mutagenesis, exchange of the enzyme's cysteine residues involved in rearrangement of disulfide bonds by function in thiol/disulfide exchange | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+ and Mg2+ have no effect on the enzyme and do not influence Zn2+ effects | Rattus norvegicus | |
Zn2+ | required for enzyme dimerization and oligomerization | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
Zn2+ induces formation of larger enzyme aggregates with MW up to 600 kDa and above | Rattus norvegicus |
52000 | - |
2 * 55000, about, sequence calculation, 2 * 52000, analytical ultrafiltration | Rattus norvegicus |
55000 | - |
2 * 55000, about, sequence calculation, 2 * 52000, analytical ultrafiltration | Rattus norvegicus |
107000 | - |
analytical ultrafiltration | Rattus norvegicus |
108000 | - |
gel filtration | Rattus norvegicus |
326000 | - |
gel filtration, in presence of 1 mM Zn2+ | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant PDI from Escherichia coli to homogeneity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ribonuclease | refolding of reduced ribonuclease in presence of glutathione, isomerase activity of PDI | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, about, sequence calculation, 2 * 52000, analytical ultrafiltration | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
PDI | - |
Rattus norvegicus |
protein disulfide isomerase | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |