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Literature summary for 5.3.4.1 extracted from

  • Solovyov, A.; Gilbert, H.F.
    Zinc-dependent dimerization of the folding catalyst, protein disulfide isomerase (2004), Protein Sci., 13, 1902-1907.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant PDI in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C294S/C325S site-directed mutagenesis, exchange of the enzyme's cysteine residues involved in rearrangement of disulfide bonds by function in thiol/disulfide exchange Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Rattus norvegicus 5783
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+ and Mg2+ have no effect on the enzyme and do not influence Zn2+ effects Rattus norvegicus
Zn2+ required for enzyme dimerization and oligomerization Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
Zn2+ induces formation of larger enzyme aggregates with MW up to 600 kDa and above Rattus norvegicus
52000
-
2 * 55000, about, sequence calculation, 2 * 52000, analytical ultrafiltration Rattus norvegicus
55000
-
2 * 55000, about, sequence calculation, 2 * 52000, analytical ultrafiltration Rattus norvegicus
107000
-
analytical ultrafiltration Rattus norvegicus
108000
-
gel filtration Rattus norvegicus
326000
-
gel filtration, in presence of 1 mM Zn2+ Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant PDI from Escherichia coli to homogeneity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced ribonuclease refolding of reduced ribonuclease in presence of glutathione, isomerase activity of PDI Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, about, sequence calculation, 2 * 52000, analytical ultrafiltration Rattus norvegicus

Synonyms

Synonyms Comment Organism
PDI
-
Rattus norvegicus
protein disulfide isomerase
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rattus norvegicus