Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.4.1 extracted from

  • Li, S.J.; Hong, X.G.; Shi, Y.Y.; Li, H.; Wang, C.C.
    Annular arrangement and collaborative actions of four domains of protein disulfide isomerase - A small angle X-ray scattering study in solution (2005), J. Biol. Chem., 281, 6581-6588.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant PDIs in Escherichia coli strain M15 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
small angle X-ray scattering analysis of purified recombinant wild-type and deletion mutant PDIs, structure modeling, the enzyme forms an approximately flat elliptical cylinder Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of several deletion mutants of PDI by elimination of partial domains or whole domains, overview Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
1 * 64000, analytical ultracentrifugation Homo sapiens
69000
-
light scattering Homo sapiens
116000
-
gel filtration, the C-terminal fragment comprising residues 441-491 contributes to the anomalous molecular mass determination by gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P07237
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant PDIs from Escherichia coli strain M15 by nickel affinity and cation exchange chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the four domains a, b, b', and a' show cooperative properties in both isomerase and chaperone functions of PDi Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64000, analytical ultracentrifugation Homo sapiens
More the four domains a, b, b', and a' are arranged in an annular manner Homo sapiens

Synonyms

Synonyms Comment Organism
PDI
-
Homo sapiens