Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GSH | required for activity | Saccharomyces cerevisiae | |
GSSG | required for activity | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type PDI and mutant PDI defective in the a' domain, which bears the isomerase activity, in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a mutant PDI defective in the a' domain, which bears the isomerase activity, a PDI deletion yeast mutant cannot be rescued by the rat PDI | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Saccharomyces cerevisiae | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxypeptidase Y | Saccharomyces cerevisiae | maturation of carboxypeptidase Y | ? | - |
? | |
additional information | Saccharomyces cerevisiae | essential enzyme for yeast cell growth, both oxidase and isomerase activities are required | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type PDI and mutant PDI defective in the a' domain from Escherichia coli by nickel affnity chromatography | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
catalyses the rearrangement of -S-S- bonds in proteins | PDI introduces disulfides into proteins, oxidase activity, and provides quality control by catalyzing the rearrangement of incorrect disulfides, isomerase activity | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxypeptidase Y | maturation of carboxypeptidase Y | Saccharomyces cerevisiae | ? | - |
? | |
additional information | essential enzyme for yeast cell growth, both oxidase and isomerase activities are required | Saccharomyces cerevisiae | ? | - |
? | |
refolding of RNase | renaturation of reduced RNase | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the catalytic domains a and a' are responsible for the oxidase and the isomerase activity, respectively | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PDI | - |
Saccharomyces cerevisiae |
protein disulfide isomerase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |