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Literature summary for 5.3.3.5 extracted from

  • Bae, S.H.; Seong, J.; Paik, Y.K.
    Cholesterol biosynthesis from lanosterol: molecular cloning, chromosomal localization, functional expression and liver-specific gene regulation of rat sterol DELTA8-isomerase, a cholesterogenic enzyme with multiple functions (2001), Biochem. J., 353, 689-699.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.3.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
3beta-[2-(diethylamino)ethoxy]androst-5-en-17-one IC50: 0.0042 mM Rattus norvegicus
trans-2-[4-(1,2-diphenylbuten-1-yl)phenoxy]-N,N-dimethylethylamine IC50: 0.0112 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26737
-
 x * 26737, calculation from nucleotide sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-cholest-7-en-3beta-ol Rattus norvegicus enzyme of the cholesterol biosynthetic pathway, mutations are associated with Conradi-Huenermann syndrome. The enzyme may be involved in liver function, since the mRNA is only detected in hepatocytes not in connective tissue or blood vessels. The expression of the SI gene is regulated transcriptionally in vitro and in vivo by widely known regulatory agents ranging from tamoxifen, an oestrogen receptor antagonist and SI-specific inhibitor, to cholesterol-lowering drugs ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q9JJ46
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-cholest-7-en-3beta-ol enzyme of the cholesterol biosynthetic pathway, mutations are associated with Conradi-Huenermann syndrome. The enzyme may be involved in liver function, since the mRNA is only detected in hepatocytes not in connective tissue or blood vessels. The expression of the SI gene is regulated transcriptionally in vitro and in vivo by widely known regulatory agents ranging from tamoxifen, an oestrogen receptor antagonist and SI-specific inhibitor, to cholesterol-lowering drugs Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 26737, calculation from nucleotide sequence Rattus norvegicus

Synonyms

Synonyms Comment Organism
SI
-
 Rattus norvegicus
sterol DELTA8 isomerase
-
 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0042
-
 IC50: 0.0042 mM Rattus norvegicus 3beta-[2-(diethylamino)ethoxy]androst-5-en-17-one
0.0112
-
 IC50: 0.0112 mM Rattus norvegicus trans-2-[4-(1,2-diphenylbuten-1-yl)phenoxy]-N,N-dimethylethylamine