Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.3.3 extracted from

  • Scherf, U.; S๖hling, B.; Gottschalk, G.; Linder, D.; Buckel, W.
    Succinate-ethanol fermentation in Clostridium kluyveri: purification, characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA DELTA3-DELTA2-isomerase (1994), Arch. Microbiol., 161, 239-245.
    View publication on PubMed
Search for more literature for 5.3.3.3

Metals/Ions

Metals/Ions Comment Organism Structure
Fe-S 0.8 mol Fe and 10.8 mol S per mol of enzyme, iron-sulfur protein, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium kluyveri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 4 * 59000, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, SDS-PAGE Clostridium kluyveri
237000
-
 bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, gel filtration Clostridium kluyveri

Organism

Organism UniProt Comment Textmining
Clostridium kluyveri
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen irreversibly inactivates Clostridium kluyveri

Purification (Commentary)

Purification (Comment) Organism
bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium kluyveri

Subunits

Subunits Comment Organism
tetramer 4 * 59000, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, SDS-PAGE Clostridium kluyveri

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol per mol of enzyme, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium kluyveri