Literature summary for 5.3.3.2 extracted from

Nakatani, H.; Goda, S.; Unno, H.; Nagai, T.; Yoshimura, T.; Hemmi, H.
Substrate-induced change in the quaternary structure of type 2 isopentenyl diphosphate isomerase from Sulfolobus shibatae (2012), J. Bacteriol., 194, 3216-3224.

Search for more literature for 5.3.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
recombinantly expressed with a polyhistidine tag at its N terminus in Escherichia coli BL21	Saccharolobus shibatae

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallized at 20°C using the hanging-drop vapor diffusion method with a reservoir solution containing 0.1 M Tris-HCl (pH 8.0), 0.2 M sodium citrate, and 30% (vol/vol) polyethylene glycol 400 (PEG 400)	Saccharolobus shibatae

Protein Variants

Protein Variants	Comment	Organism
E13R/R235E	the mutant and the wild-type enzyme show similar Vmax values, while the Km of E13R/R235E is smaller than that of the wild type. The mutant is in the tetrameric state even at a concentration where the wild-type enzyme dominantly forms an octamer	Saccharolobus shibatae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00714	-	isopentenyl diphosphate	pH 6.0, 60°C, mutant enzyme E13R/R235E	Saccharolobus shibatae
0.0463	-	isopentenyl diphosphate	pH 6.0, 60°C, wild-type enzyme	Saccharolobus shibatae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isopentenyl diphosphate	Saccharolobus shibatae	-	dimethylallyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus shibatae	P61615	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isopentenyl diphosphate	-	Saccharolobus shibatae	dimethylallyl diphosphate	-	?

Subunits

Subunits	Comment	Organism
octamer	substrate binding causes the dissociation of an octamer into tetramers. The mutant enzyme E13R/R235E is in the tetrameric state even at a concentration where the wild-type enzyme dominantly forms an octamer	Saccharolobus shibatae

Synonyms

Synonyms	Comment	Organism
type 2 isopentenyl diphosphate isomerase	-	Saccharolobus shibatae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Saccharolobus shibatae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Saccharolobus shibatae

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Release 2023.1 (January 2023)