Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.3.2 extracted from

  • Unno, H.; Yamashita, S.; Ikeda, Y.; Sekiguchi, S.Y.; Yoshida, N.; Yoshimura, T.; Kusunoki, M.; Nakayama, T.; Nishino, T.; Hemmi, H.
    New role of flavin as a general acid-base catalyst with no redox function in type 2 isopentenyl-diphosphate isomerase (2009), J. Biol. Chem., 284, 9160-9167.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.3.2

Cloned(Commentary)

Cloned (Comment) Organism
into a pET-vector for expression in Escherichia coli Bl21DE3 cells Saccharolobus shibatae

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structures of the substrate-free enzyme and of the substrate-enzyme complexes, in the oxidized and reduced states, are solved to resolutions between 1.99 and 3.1 A, six distinct types of type 2 IDI crystals are obtained Saccharolobus shibatae

Protein Variants

Protein Variants Comment Organism
D216A mutation of highly conserved residue Saccharolobus shibatae
E161A mutation of highly conserved residue Saccharolobus shibatae
E194A mutation of highly conserved residue Saccharolobus shibatae
E229A mutation of highly conserved residue Saccharolobus shibatae
H11A mutation of highly conserved residue Saccharolobus shibatae
H155A mutation of highly conserved residue Saccharolobus shibatae
K193A mutation of highly conserved residue Saccharolobus shibatae
K8A mutation of highly conserved residue Saccharolobus shibatae
N125A mutation of highly conserved residue Saccharolobus shibatae
N157A mutation of highly conserved residue Saccharolobus shibatae
Q160A mutation of highly conserved residue Saccharolobus shibatae
R232A mutation of highly conserved residue Saccharolobus shibatae
R7A mutation of highly conserved residue Saccharolobus shibatae
S96A mutation of highly conserved residue Saccharolobus shibatae
T68A mutation of highly conserved residue Saccharolobus shibatae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharolobus shibatae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isopentenyl diphosphate Saccharolobus shibatae
-
 dimethylallyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae P61615
-
-

Purification (Commentary)

Purification (Comment) Organism
on a HisTrap column, for crystallisation, the enzyme is loaded on a HiLoad 16/60 Superdex 200 column Saccharolobus shibatae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 mutant D216A, relative IPP isomerase activity 35%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant E161A, relative IPP isomerase activity 0.5%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant E194A, relative IPP isomerase activity 15%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant E229A, relative IPP isomerase activity 6.5%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant H11A, relative IPP isomerase activity 4%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant H155A, relative IPP isomerase activity 3%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant K193A, relative IPP isomerase activity 1%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant K8A, relative IPP isomerase activity 0.1%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant N125A, relative IPP isomerase activity 5%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant N157A, relative IPP isomerase activity 0.5%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant Q160A, relative IPP isomerase activity 0.1%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant R232A, relative IPP isomerase activity 2%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant R7A, relative IPP isomerase activity 0.1%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant S96A, relative IPP isomerase activity 3%, wild-type enzyme 100% Saccharolobus shibatae
additional information
-
 mutant T68A, relative IPP isomerase activity 6%, wild-type enzyme 100% Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate
-
 Saccharolobus shibatae dimethylallyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
IDI
-
 Saccharolobus shibatae
IPP isomerase
-
 Saccharolobus shibatae
isopentenyl diphosphate:dimethylallyl diphosphate isomerase
-
 Saccharolobus shibatae
type 2 isopentenyl-diphosphate isomerase
-
 Saccharolobus shibatae

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Saccharolobus shibatae
NAD(P)H used only for the reduction of FMN, can be replaced with Na2S2O4 Saccharolobus shibatae