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Literature summary for 5.3.3.2 extracted from
- Enzymatic and structural characterization of type II isopentenyl diphosphate isomerase from hyperthermophilic archaeon Thermococcus kodakaraensis (2005), Biochem. Biophys. Res. Commun., 331, 1127-1136.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21(DE3) | Thermococcus kodakarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
isopentenyl diphosphate | pH 8.0, 50°C | Thermococcus kodakarensis |  |
| 0.047 | - |
isopentenyl diphosphate | pH 8.0, 55°C | Thermococcus kodakarensis | |
| 0.063 | - |
isopentenyl diphosphate | pH 8.0, 60°C | Thermococcus kodakarensis | |
| 0.065 | - |
isopentenyl diphosphate | pH 8.0, 70°C | Thermococcus kodakarensis | |
| 0.071 | - |
isopentenyl diphosphate | pH 8.0, 65°C | Thermococcus kodakarensis | |
| 0.077 | - |
isopentenyl diphosphate | pH 8.0, 75°C | Thermococcus kodakarensis | |
| 0.084 | - |
isopentenyl diphosphate | pH 8.0, 80°C | Thermococcus kodakarensis | |
| 0.093 | - |
isopentenyl diphosphate | pH 8.0, 90°C | Thermococcus kodakarensis | |
| 0.187 | - |
isopentenyl diphosphate | pH 8.0, 82.5°C | Thermococcus kodakarensis | |
| 0.199 | - |
isopentenyl diphosphate | pH 8.0, 87.5°C | Thermococcus kodakarensis | |
| 0.211 | - |
isopentenyl diphosphate | pH 8.0, 85°C | Thermococcus kodakarensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Thermococcus kodakarensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41492 | - |
8 * 41492, calculated | Thermococcus kodakarensis |
| 345000 | - |
gel filtration chromatography | Thermococcus kodakarensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isopentenyl diphosphate | - |
Thermococcus kodakarensis | dimethylallyl diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 41492, calculated | Thermococcus kodakarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDI | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
maximum activity | Thermococcus kodakarensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 90 | - |
Thermococcus kodakarensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
1h, IDI retained 50% of the activity | Thermococcus kodakarensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Thermococcus kodakarensis | |
| NADPH | - |
Thermococcus kodakarensis | |
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