Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(dimethylamino)ethyl phosphate | - |
Cinchona robusta | |
geranyl diphosphate | - |
Cinchona robusta | |
Mg2+ | isomerase I is activated by concentrations below 1 mM of Mn2+ or Mg2+, increasing concentrations are inhibitory | Cinchona robusta | |
Mn2+ | isomerase I is activated by concentrations below 1 mM of Mn2+ or Mg2+, increasing concentrations are inhibitory | Cinchona robusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
isopentenyl diphosphate | isomerase II | Cinchona robusta | |
0.0051 | - |
isopentenyl diphosphate | isomerase I | Cinchona robusta | |
0.017 | - |
dimethylallyl diphosphate | isomerase I | Cinchona robusta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mn2+ or Mg2+ required | Cinchona robusta | |
Mg2+ | activation by a mixture of Mg2+ and Mn2+ gives the highest activity | Cinchona robusta | |
Mg2+ | isomerase I is activated by concentrations below 1 mM of Mn2+ or Mg2+, increasing concentrations are inhibitory | Cinchona robusta | |
Mg2+ | isomerase II: maximal activation at 2-8 mM Mg2+, the maximal activation is 35% lower than the activation obtained with Mn2+ | Cinchona robusta | |
Mn2+ | Mn2+ or Mg2+ required | Cinchona robusta | |
Mn2+ | activation by a mixture of Mg2+ and Mn2+ gives the highest activity | Cinchona robusta | |
Mn2+ | isomerase I is activated by concentrations below 1 mM of Mn2+ or Mg2+, increasing concentrations are inhibitory | Cinchona robusta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, isomerase I, SDS-PAGE | Cinchona robusta |
34000 | - |
x * 34000, isomerase II, SDS-PAGE | Cinchona robusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cinchona robusta | induced after addition of a homogenate of the phytopathogenic fungus Phytophthora cinnamomi. Enzyme may be involved in the biosynthesis of anthraquinone phytoalexins that accumulate rapidly after elicitation of Cinchona cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cinchona robusta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2 isoforms: I and II | Cinchona robusta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Cinchona robusta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.046 | - |
- |
Cinchona robusta |
Storage Stability | Organism |
---|---|
-80°C, 0.05 M Tris/HCl, pH 7.5, containing 0.01 mM leupeptin, 2 mM dithiothreitol, 5% glycerol, 55% of the activity is recovered after 3 months | Cinchona robusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | r | Cinchona robusta | dimethylallyl diphosphate | - |
? | |
additional information | induced after addition of a homogenate of the phytopathogenic fungus Phytophthora cinnamomi. Enzyme may be involved in the biosynthesis of anthraquinone phytoalexins that accumulate rapidly after elicitation of Cinchona cells | Cinchona robusta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, isomerase I, SDS-PAGE | Cinchona robusta |
? | x * 34000, isomerase II, SDS-PAGE | Cinchona robusta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7.0-7.8: maximal activity, pH 8.5: 20% of maximal activity, isomerase I | Cinchona robusta |