General Stability | Organism |
---|---|
high susceptibility to proteolysis | Claviceps sp. |
unstable in dilute solutions | Claviceps sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-cis,6,7-trans-farnesyl diphosphate | - |
Gallus gallus | |
3-methyl-5-oxo-hexyl diphosphate | - |
Gallus gallus | |
3-methyl-5-oxo-hexyl diphosphate ethylene glycol acetal | - |
Gallus gallus | |
dimethylallyl diphosphate | - |
Gallus gallus | |
diphosphate | - |
Claviceps sp. | |
diphosphate | - |
Gallus gallus | |
farnesyl diphosphate | - |
Claviceps sp. | |
farnesyl diphosphate | - |
Gallus gallus | |
geranyl diphosphate | - |
Claviceps sp. | |
geranyl diphosphate | - |
Gallus gallus | |
iodoacetic acid | - |
Claviceps sp. | |
Octyl diphosphate | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
isopentenyl diphosphate | - |
Claviceps sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Gallus gallus | |
Mg2+ | Mn2+ or Mg2+ required | Claviceps sp. | |
Mg2+ | maximal activation at 0.2 mM | Claviceps sp. | |
Mn2+ | - |
Gallus gallus | |
Mn2+ | Mn2+ or Mg2+ required | Claviceps sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Claviceps sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Claviceps sp. | - |
- |
- |
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Claviceps sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3000 | - |
- |
Claviceps sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Gallus gallus | dimethylallyl diphosphate | - |
? | |
isopentenyl diphosphate | - |
Claviceps sp. | dimethylallyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Claviceps sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life in dilute solution: 30 min. In more concentrated solutions of the protein or dilute enzyme in the presence of 0.01% bovine serum albumin, little loss of activity after 1 h | Claviceps sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | in presence of Mg2+. A sharper maximum is observed in presence of Mn2+ | Claviceps sp. |