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Literature summary for 5.3.3.2 extracted from

  • Bruenger, E.; Chayet, L.; Rilling, H.C.
    Isopentenyl pyrophosphate isomerase:dimetylallyl pyrophosphate isomerase: isolation from Claviceps sp. SD 58 and Comparison to the mammalian enzyme (1986), Arch. Biochem. Biophys., 248, 620-625.
    View publication on PubMed

General Stability

General Stability Organism
high susceptibility to proteolysis Claviceps sp.
unstable in dilute solutions Claviceps sp.

Inhibitors

Inhibitors Comment Organism Structure
2,3-cis,6,7-trans-farnesyl diphosphate
-
Gallus gallus
3-methyl-5-oxo-hexyl diphosphate
-
Gallus gallus
3-methyl-5-oxo-hexyl diphosphate ethylene glycol acetal
-
Gallus gallus
dimethylallyl diphosphate
-
Gallus gallus
diphosphate
-
Claviceps sp.
diphosphate
-
Gallus gallus
farnesyl diphosphate
-
Claviceps sp.
farnesyl diphosphate
-
Gallus gallus
geranyl diphosphate
-
Claviceps sp.
geranyl diphosphate
-
Gallus gallus
iodoacetic acid
-
Claviceps sp.
Octyl diphosphate
-
Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
isopentenyl diphosphate
-
Claviceps sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Gallus gallus
Mg2+ Mn2+ or Mg2+ required Claviceps sp.
Mg2+ maximal activation at 0.2 mM Claviceps sp.
Mn2+
-
Gallus gallus
Mn2+ Mn2+ or Mg2+ required Claviceps sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Claviceps sp.

Organism

Organism UniProt Comment Textmining
Claviceps sp.
-
-
-
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Claviceps sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3000
-
-
Claviceps sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate
-
Gallus gallus dimethylallyl diphosphate
-
?
isopentenyl diphosphate
-
Claviceps sp. dimethylallyl diphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Claviceps sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
half-life in dilute solution: 30 min. In more concentrated solutions of the protein or dilute enzyme in the presence of 0.01% bovine serum albumin, little loss of activity after 1 h Claviceps sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.5 in presence of Mg2+. A sharper maximum is observed in presence of Mn2+ Claviceps sp.