BRENDA - Enzyme Database
show all sequences of 5.3.3.13

On the mechanism of a polyunsaturated fatty acid double bond isomerase from Propionibacterium acnes

Liavonchanka, A.; Rudolph, M.G.; Tittmann, K.; Hamberg, M.; Feussner, I.; J. Biol. Chem. 284, 8005-8012 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
into the vector pGEX-6P-1 for expression in Escherichia coli cells
Cutibacterium acnes
Engineering
Protein Variants
Commentary
Organism
F168G/G169F
inactive mutant
Cutibacterium acnes
F168L
inactive mutant
Cutibacterium acnes
F193A
mutant
Cutibacterium acnes
R88A
inactive mutant
Cutibacterium acnes
R88S
mutant
Cutibacterium acnes
R88S/F193A
mutant
Cutibacterium acnes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
(11S-2H) linoleic acid, 0.00408, wild-type PAI
Cutibacterium acnes
additional information
-
additional information
[11,11-2H2] linoleic acid, 0.00228, wild-type PAI
Cutibacterium acnes
0.00482
-
linoleic acid
wild-type PAI
Cutibacterium acnes
0.02577
-
linoleic acid
mutant R88S/F193A
Cutibacterium acnes
0.03794
-
linoleic acid
mutant F193A
Cutibacterium acnes
0.0657
-
linoleic acid
mutant R88S
Cutibacterium acnes
Organism
Organism
UniProt
Commentary
Textmining
Cutibacterium acnes
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Cutibacterium acnes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
linoleic acid
-
704631
Cutibacterium acnes
?
-
-
-
?
linoleic acid methyl ester
-
704631
Cutibacterium acnes
(10E,12Z)-octadeca-10,12-dienoic acid methyl ester
-
-
-
?
linoleoyl-CoA
-
704631
Cutibacterium acnes
(10Z,12Z)-octadeca-10,12-dienoyl-CoA
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
PAI
-
Cutibacterium acnes
polyunsaturated fatty acid double bond isomerase
-
Cutibacterium acnes
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
kinetic assay
Cutibacterium acnes
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
(11S-2H) linoleic acid, 3.51, wild-type PAI
Cutibacterium acnes
additional information
-
additional information
[11,11-2H2] linoleic acid, 0.83, wild-type PAI
Cutibacterium acnes
0.16
-
linoleic acid
mutant R88S/F193A
Cutibacterium acnes
0.33
-
linoleic acid
mutant F193A
Cutibacterium acnes
2.93
-
linoleic acid
mutant R88S
Cutibacterium acnes
4.37
-
linoleic acid
wild-type PAI
Cutibacterium acnes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
activity and kinetic assay
Cutibacterium acnes
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Cutibacterium acnes
Cloned(Commentary) (protein specific)
Commentary
Organism
into the vector pGEX-6P-1 for expression in Escherichia coli cells
Cutibacterium acnes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Cutibacterium acnes
Engineering (protein specific)
Protein Variants
Commentary
Organism
F168G/G169F
inactive mutant
Cutibacterium acnes
F168L
inactive mutant
Cutibacterium acnes
F193A
mutant
Cutibacterium acnes
R88A
inactive mutant
Cutibacterium acnes
R88S
mutant
Cutibacterium acnes
R88S/F193A
mutant
Cutibacterium acnes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
(11S-2H) linoleic acid, 0.00408, wild-type PAI
Cutibacterium acnes
additional information
-
additional information
[11,11-2H2] linoleic acid, 0.00228, wild-type PAI
Cutibacterium acnes
0.00482
-
linoleic acid
wild-type PAI
Cutibacterium acnes
0.02577
-
linoleic acid
mutant R88S/F193A
Cutibacterium acnes
0.03794
-
linoleic acid
mutant F193A
Cutibacterium acnes
0.0657
-
linoleic acid
mutant R88S
Cutibacterium acnes
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cutibacterium acnes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
linoleic acid
-
704631
Cutibacterium acnes
?
-
-
-
?
linoleic acid methyl ester
-
704631
Cutibacterium acnes
(10E,12Z)-octadeca-10,12-dienoic acid methyl ester
-
-
-
?
linoleoyl-CoA
-
704631
Cutibacterium acnes
(10Z,12Z)-octadeca-10,12-dienoyl-CoA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
kinetic assay
Cutibacterium acnes
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
(11S-2H) linoleic acid, 3.51, wild-type PAI
Cutibacterium acnes
additional information
-
additional information
[11,11-2H2] linoleic acid, 0.83, wild-type PAI
Cutibacterium acnes
0.16
-
linoleic acid
mutant R88S/F193A
Cutibacterium acnes
0.33
-
linoleic acid
mutant F193A
Cutibacterium acnes
2.93
-
linoleic acid
mutant R88S
Cutibacterium acnes
4.37
-
linoleic acid
wild-type PAI
Cutibacterium acnes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
activity and kinetic assay
Cutibacterium acnes
Other publictions for EC 5.3.3.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704631
Liavonchanka
On the mechanism of a polyunsa ...
Cutibacterium acnes
J. Biol. Chem.
284
8005-8012
2009
-
-
1
-
6
-
-
6
-
-
-
-
-
4
-
-
1
-
-
-
-
-
3
-
2
1
-
-
6
1
-
-
1
-
-
-
-
-
1
1
-
6
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
-
-
-
-
-
691788
Liavonchanka
Biochemistry of PUFA double bo ...
Ptilota filicina
ChemBioChem
9
1867-1872
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649079
Zheng
Polyenoic fatty-acid isomerase ...
Ptilota filicina
Arch. Biochem. Biophys.
402
11-20
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654340
Zheng
Polyenoic fatty acid isomerase ...
Ptilota filicina
Arch. Biochem. Biophys.
401
11-20
2002
-
-
1
-
-
-
-
-
-
-
4
1
-
6
-
2
1
-
-
-
-
1
4
1
-
1
-
-
-
1
-
-
1
-
2
-
-
-
1
1
-
-
-
-
-
-
-
-
-
4
1
-
-
2
1
-
-
-
1
4
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
649078
Wise
Binding site characterization ...
Ptilota filicina
Biochemistry
36
2985-2992
1997
-
-
-
-
-
-
-
9
-
-
1
-
-
4
-
-
1
1
-
-
1
1
10
1
-
1
-
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
1
-
-
1
1
10
1
1
-
-
1
1
1
1
-
-
-
-
-
-
-
654153
Gerwick
Biosynthesis and cannabinoid r ...
Ptilota filicina
Adv. Exp. Med. Biol.
407
329-334
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
649077
Wise
Synthesis and cannabinoid rece ...
Ptilota filicina
Experientia
52
88-92
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
649076
Wise
Biosynthesis of conjugated fat ...
Ptilota filicina
Biochemistry
33
15223-15232
1994
-
-
-
-
-
-
1
3
-
-
3
1
-
4
-
-
1
1
-
-
1
-
9
1
-
-
-
-
1
1
1
-
1
-
1
-
-
-
-
1
-
-
-
-
1
-
3
-
-
3
1
-
-
-
1
-
-
1
-
9
1
-
-
-
1
1
1
-
1
-
-
-
-
-
-