Protein Variants | Comment | Organism |
---|---|---|
D40N | inhibition compared to wild-type enzyme, overview | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-dihydroxynaphthalene | - |
Pseudomonas putida | |
6-bromo-2-naphthol | - |
Pseudomonas putida | |
6-cyano-2-naphthol | - |
Pseudomonas putida | |
6-methoxy-2-naphthol | - |
Pseudomonas putida | |
equilenin | - |
Pseudomonas putida | |
additional information | inhibitor development, a series of substituted naphthol inhibitors shows substantial variations in the contributions of the protonated and deprotonated forms when bound to the active site. Method of quantifying the contributions of each protonation state show the oxyanion hole in the active site of wild-type DELTA5-3-ketosteroid isomerase to have a proton affinity equal to a solution pKa of 10.05, which is similar to the measured pKa of 10.0 of the reaction intermediate | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
DELTA5-3-ketosteroid isomerase | - |
Pseudomonas putida |
ketosteroid isomerase | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dissociation constants of inhibitors | Pseudomonas putida |