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Literature summary for 5.3.3.1 extracted from

  • Sharma, K.; Vazquez-Ramirez, R.; Kubli-Garfias, C.
    A theoretical model of the catalytic mechanism of the DELTA5-3-ketosteroid isomerase reaction (2006), Steroids, 71, 549-557.
    View publication on PubMed
Search for more literature for 5.3.3.1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-Androstene-3,17-dione Pseudomonas sp.
-
 4-Androstene-3,17-dione
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a 3-oxo-DELTA5-steroid = a 3-oxo-DELTA4-steroid two-step reaction first, Asp38 acts as a base, abstracting the 4beta-H atom from C-4 of the steroid to form a dienolate as the intermediate, next, the intermediate is reketonized by proton transfer to the 6beta-position, each step goes through its own transition state, catalytic mechanism, theoretical model involving residues Tyr14, Asp38 and Asp99 Pseudomonas sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-Androstene-3,17-dione
-
 Pseudomonas sp. 4-Androstene-3,17-dione
-
 ?

Synonyms

Synonyms Comment Organism
DELTA5-3-ketosteroid isomerase
-
 Pseudomonas sp.
KSI
-
 Pseudomonas sp.