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Literature summary for 5.3.3.1 extracted from

  • Houck, W.J.; Pollack, R.M.
    Temperature effects on the catalytic activity of the D38E mutant of 3-oxo-DELTA5-steroid isomerase: favorable enthalpies and entropies of activation relative to the nonenzymatic reaction catalyzed by acetate ion (2004), J. Am. Chem. Soc., 126, 16416-16425.
    View publication on PubMed
Search for more literature for 5.3.3.1

Protein Variants

Protein Variants Comment Organism
D38E compared to wild-type, 200fold reduction in kcat-value. Analysis of thermodynamic parameters Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information thermodynamic parameters and rate constants for isomerization of 5-androstene-3,17-dione Comamonas testosteroni
0.033
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 5.4 °C Comamonas testosteroni
0.037
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 10.3 °C Comamonas testosteroni
0.04
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 15.3 °C Comamonas testosteroni
0.04
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 20.1 °C Comamonas testosteroni
0.055
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 25.0 °C Comamonas testosteroni
0.063
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 29.9 °C Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni P00947
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-Androstene-3,17-dione
-
 Comamonas testosteroni 4-Androstene-3,17-dione
-
 ?

Synonyms

Synonyms Comment Organism
3-oxo-DELTA5-steroid isomerase
-
 Comamonas testosteroni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
53
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 5.4 °C Comamonas testosteroni
71
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 10.3 °C Comamonas testosteroni
101
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 15.3 °C Comamonas testosteroni
145
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 20.1 °C Comamonas testosteroni
180
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 25.0 °C Comamonas testosteroni
250
-
 5-androstene-3,17-dione mutant D38E, pH 7.0, 29.9 °C Comamonas testosteroni