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Literature summary for 5.3.3.1 extracted from

  • Kim, S.W.; Choi, K.Y.
    Identification of active site residues by site-directed mutagenesis of DELTA5-3-ketosteroid isomerase from Pseudomonas putida biotype B (1995), J. Bacteriol., 177, 2602-2605.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes D40N and Y16F in Escherichia col BL21 Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
D40N 1484555fold decrease in turnover number, 4.46fold decrease in KM-value as compared to wild-type enzyme Pseudomonas putida
Y16F 2009fold decrease in turnover number, 3.5fold decrease in KM-value as compared to wild-type enzyme Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values of mutant enzymes Y16F and D40N Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14520
-
 mutant enzyme D40N, electrospray mass spectrometry Pseudomonas putida
14520
-
 mutant enzyme Y16F, electrospray mass spectrometry Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 biotype B
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes D40N and Y16F Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover number of mutant enzymes Y16F and D40N Pseudomonas putida