Cloned (Comment) | Organism |
---|---|
insect Sf9 cells transfected with recombinant baculovirus | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method. The inability to crystallize the detergent-solubilized, wild-type microsomal enzyme is overcome by engineering a cytosolic form of this protein. Modified enzyme in which the membrane-spanning domain, residues 283-310 of the enzyme protein is deleted in the cDNA is expressed by baculovirus in the cytosol instead of in the microsomes and mitochondria of the Sf9 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTA283-310 | the molecular weight of the subunit is 38800 Da compared to 42000 Da for the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0252 | - |
5-androstene-3,17-dione | 22°C, pH 7.4, truncated cytosolic enzyme DEALTA283-310 | Homo sapiens | |
0.0279 | - |
5-androstene-3,17-dione | 22°C, pH 7.4, wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | modified enzyme in which the membrane-spanning domain, residues 283-310, of the enzyme protein is deleted in the cDNA is expressed by baculovirus in the cytosol instead of in the microsomes and mitochondria of the Sf9 cells | Homo sapiens | 5829 | - |
membrane | integral membrane protein | Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38800 | - |
x * 38800, truncated soluble enzyme DEALTA283-310 | Homo sapiens |
42000 | - |
x * 42000, wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
modified enzyme in which the membrane-spanning domain, residues 283-310, of the enzyme protein is deleted in the cDNA is expressed by baculovirus in the cytosol instead of in the microsomes and mitochondria of the Sf9 cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.622 | - |
reaction with 5-androsten-3,17-dione, truncated cytosolic enzyme DELTA283-310 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-Androstene-3,17-dione | - |
Homo sapiens | 4-Androstene-3,17-dione | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38800, truncated soluble enzyme DEALTA283-310 | Homo sapiens |
? | x * 42000, wild-type enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the isomerase activity of the wild-type enzyme and the deletion mutant enzyme DELTA283-310 requires allosteric activation by NADH, Km for wild-type enzyme and deletion mutant enzyme is 0.0046 mM | Homo sapiens |