Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.3.1 extracted from

  • Kawahara, F.S.; Wang, S.F.; Talalay, P.
    The preparation and properties of crystalline DELTA5-3-ketosteroid isomerase (1962), J. Biol. Chem., 237, 1500-1506.
    View publication on PubMed
Search for more literature for 5.3.3.1

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Comamonas testosteroni

Inhibitors

Inhibitors Comment Organism Structure
17beta-estradiol competitive Comamonas testosteroni
Urea noncompetitive Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0223
-
 17beta-Hydroxy-5(10)-estren-3-one
-
 Comamonas testosteroni
0.036
-
 5-androstene-3,17-dione
-
 Comamonas testosteroni
0.31 0.33 5-androstene-3,17-dione
-
 Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40800
-
 low speed sedimentation without reaching equilibrium Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17beta-Hydroxy-5(10)-estren-3-one at 0.25% of the activity compared to 5-androstene-3,17-dione Comamonas testosteroni ?
-
 ?
5-Androstene-3,17-dione
-
 Comamonas testosteroni 4-Androstene-3,17-dione
-
 ?
5-Pregnene-3,20-dione
-
 Comamonas testosteroni 4-Pregnene-3,20-dione
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
70.8
-
 17beta-Hydroxy-5(10)-estren-3-one
-
 Comamonas testosteroni
288000
-
 5-androstene-3,17-dione
-
 Comamonas testosteroni