Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.2.7 extracted from

  • Claesson, M.; Lindqvist, Y.; Madrid, S.; Sandalova, T.; Fiskesund, R.; Yu, S.; Schneider, G.
    Crystal structure of bifunctional aldos-2-ulose dehydratase/isomerase from Phanerochaete chrysosporium with the reaction intermediate ascopyrone M (2012), J. Mol. Biol., 417, 279-293.
    View publication on PubMed
Search for more literature for 5.3.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Hansenula polymorpha Phanerodontia chrysosporium

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.0), 0.2 M MgCl2 and 16% (w/v) polyethylene glycol 6000 Phanerodontia chrysosporium

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98700
-
 2 * 98700, SDS-PAGE Phanerodontia chrysosporium
98746
-
 2 * 98746, calculated from amino acid sequence Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ascopyrone M Phanerodontia chrysosporium
-
 microthecin
-
 ?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium P84193 cf. EC 4.2.1.110
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ascopyrone M
-
 Phanerodontia chrysosporium microthecin
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 98700, SDS-PAGE Phanerodontia chrysosporium
homodimer 2 * 98746, calculated from amino acid sequence Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
AUDH bifunctional enzyme with EC 4.2.1.110 D-arabino-hex-2-ulose dehydratase and ascopyrone M isomerase activities Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 for microthecin formation Phanerodontia chrysosporium

pI Value

Organism Comment pI Value Maximum pI Value
Phanerodontia chrysosporium isoelectric focusing 5.3 5.2
Phanerodontia chrysosporium calculated from amino acid sequence
-
 5.5