Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-hexenedioate | Pseudomonas putida | ketonization process via dienol intermediate 2-hydroxymuconate and with Pro1 as a general base, one-proton transfer mechanism | 2-oxo-3-hexenedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate | catalytic reaction mechanism, detailed overview. Quantum mechanics/molecular mechanics simulation of proton transfer reaction and structure-function analysis, the ketonization mechanism in 4-OT depends on the enzymatic model used, detailed overview | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-hexenedioate | ketonization process via dienol intermediate 2-hydroxymuconate and with Pro1 as a general base, one-proton transfer mechanism | Pseudomonas putida | 2-oxo-3-hexenedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 4-OT has an amino-terminal proline and a beta-alpha-beta fold, three structural models of 4-OT, overview | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
4-OT | - |
Pseudomonas putida |
4-oxalocrotonate tautomerase | - |
Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
evolution | 4-oxalocrotonate tautomerase is a member of the tautomerase superfamily | Pseudomonas putida |
metabolism | 4-oxalocrotonate tautomerase is an essential enzyme in the degradative metabolism pathway occurring in the Krebs cycle | Pseudomonas putida |
physiological function | 4-OT catalyzes the ketonization process of 2-oxo-4-hexenedioate to its conjugated isomer, 2-oxo-3-hexadienedioate, through the dienol intermediate 2-hydroxymuconate. This proton transfer process is an essential part of degradative metabolism pathway to convert various aromatic hydrocarbons into their corresponding intermediates in the Krebs cycle | Pseudomonas putida |