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Literature summary for 5.3.2.4 extracted from
- Characterization of TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from the D-mycaminose biosynthetic pathway of Streptomyces fradiae: in vitro activity and substrate specificity studies (2007), Biochemistry, 46, 577-590.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces fradiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | pH 7.5, 25°C | Streptomyces fradiae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18806 | - |
2 * 18806, calculated from sequence | Streptomyces fradiae |
| 19000 | - |
2 * 19000, SDS-PAGE | Streptomyces fradiae |
| 31000 | - |
gel filtration | Streptomyces fradiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-alpha-D-glucose | Streptomyces fradiae | the enzyme is involved in the D-mycaminose biosynthetic pathway | dTDP-3-dehydro-6-deoxy-alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces fradiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces fradiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-4-dehydro-6-deoxy-alpha-D-glucose | efficiency of conversion is low, requiring a high concentration of enzyme and a long incubation time to achieve significant turnover | Streptomyces fradiae | CDP-3-dehydro-6-deoxy-alpha-D-glucose | - |
? | |
| dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose | efficiency is comparable to that with dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose | Streptomyces fradiae | dTDP-3-dehydro-2,6-dideoxy-alpha-D-glucose | - |
? | |
| dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | - |
Streptomyces fradiae | dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose | - |
? | |
| dTDP-4-dehydro-6-deoxy-alpha-D-glucose | the enzyme is involved in the D-mycaminose biosynthetic pathway | Streptomyces fradiae | dTDP-3-dehydro-6-deoxy-alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 19000, SDS-PAGE | Streptomyces fradiae |
| dimer | 2 * 18806, calculated from sequence | Streptomyces fradiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase | - |
Streptomyces fradiae |
| Tyl1a | - |
Streptomyces fradiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Streptomyces fradiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
CDP-4-dehydro-6-deoxy-alpha-D-glucose | pH 7.5, 25°C | Streptomyces fradiae | |
| 0.1 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | pH 7.5, 25°C | Streptomyces fradiae | |
| 0.13 | - |
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose | pH 7.5, 25°C | Streptomyces fradiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay | Streptomyces fradiae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.7 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | pH 7.5, 25°C | Streptomyces fradiae | |
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