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Literature summary for 5.3.2.3 extracted from
- Characterization of the TDP-D-ravidosamine biosynthetic pathway: one-pot enzymatic synthesis of TDP-D-ravidosamine from thymidine-5-phosphate and glucose-1-phosphate (2011), Org. Biomol. Chem., 9, 1799-1808.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | synthesis of TDP-D-ravidosamine, necessary for in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities | Streptomyces ravidus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces ravidus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19100 | - |
2 * 19100, SDS-PAGE | Streptomyces ravidus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | Streptomyces ravidus | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces ravidus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces ravidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | - |
Streptomyces ravidus | ? | - |
? | |
| dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | - |
Streptomyces ravidus | ? | no characterization of the product due to its low yield | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 19100, SDS-PAGE | Streptomyces ravidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RavIM | - |
Streptomyces ravidus |
| TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase | - |
Streptomyces ravidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptomyces ravidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Streptomyces ravidus |
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Release 2023.1 (January 2023)
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