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Literature summary for 5.3.2.1 extracted from
- Phenylpyruvate tautomerase activity of trans-3-chloroacrylic acid dehalogenase: evidence for an enol intermediate in the dehalogenase reaction? (2007), Biochemistry, 46, 9596-9604.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E52Q | mutation in alpha-subunit, 1.6fold increase in ratio of kcat to Km value | Pseudomonas pavonaceae |
| P1A | mutation in beta-subunit, 8fold decrease in ratio of kcat to Km value | Pseudomonas pavonaceae |
| R11A | mutation in alpha-subunit, 15fold decrease in ratio of kcat to Km value | Pseudomonas pavonaceae |
| R8A | mutation in alpha-subunit, 19fold decrease in ratio of kcat to Km value | Pseudomonas pavonaceae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
enol-phenylpyruvate | pH 9.0 | Mus musculus |  |
| 0.031 | - |
enol-phenylpyruvate | pH 6.8 | Mus musculus | |
| 0.061 | - |
enol-phenylpyruvate | wild-type, pH 9.0 | Pseudomonas pavonaceae | |
| 0.113 | - |
enol-phenylpyruvate | mutant E52Q, pH 9.0 | Pseudomonas pavonaceae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
macrophage migration inhibitory factor MIF | - |
| Pseudomonas pavonaceae | - |
trans-3-chloroacrylic acid dehydrogenase CaaD has phenylpyruvate tautomerase activity | - |
| Pseudomonas pavonaceae 170 | - |
trans-3-chloroacrylic acid dehydrogenase CaaD has phenylpyruvate tautomerase activity | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| enol-phenylpyruvate | - |
Mus musculus | keto-phenylpyruvate | reaction is stereoselective, generating the (3R) isomer of [3-D]phenylpyruvate in D2O in a ratio of 6.6:1 | ? | |
| enol-phenylpyruvate | reaction is stereoselective, generating the 3S isomer of [3-D]phenylpyruvate in a 18:1 ratio in D2O | Pseudomonas pavonaceae | keto-phenylpyruvate | - |
? | |
| enol-phenylpyruvate | reaction is stereoselective, generating the 3S isomer of [3-D]phenylpyruvate in a 18:1 ratio in D2O | Pseudomonas pavonaceae 170 | keto-phenylpyruvate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CaaD | - |
Pseudomonas pavonaceae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
enol-phenylpyruvate | wild-type, pH 9.0 | Pseudomonas pavonaceae | |
| 4.1 | - |
enol-phenylpyruvate | mutant E52Q, pH 9.0 | Pseudomonas pavonaceae | |
| 33 | - |
enol-phenylpyruvate | pH 9.0 | Mus musculus | |
| 38 | - |
enol-phenylpyruvate | pH 6.8 | Mus musculus | |
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Release 2023.1 (January 2023)
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