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Literature summary for 5.3.2.1 extracted from

  • Lubetsky, J.B.; Swope, M.; Dealwis, C.; Blake, P.; Lolis, E.
    Pro-1 of macrophage migration inhibitory factor functions as a catalytic base in the phenylpyruvate tautomerase activity (1999), Biochemistry, 38, 7346-7354.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, crystal structure of the enzyme complexed with p-hydroxyphenylpyruvate Homo sapiens

Protein Variants

Protein Variants Comment Organism
P1(A)M2 insertion abolishes activity Homo sapiens
P1G mutation substantially reduces the catalytic activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12345
-
x * 12345, wild-type enzyme, mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens important immunoregulatory molecule with a unique ability to suppress the anti-inflammatory effects of glucocorticoids ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14174
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
keto-(p-hydroxyphenyl)pyruvate the substrate interacts with Pro1, Lys32 and Ile64 from one subunit and Tyr95 and Asn97 from an adjacent subunit Homo sapiens enol-(p-hydroxyphenyl)pyruvate
-
?
additional information important immunoregulatory molecule with a unique ability to suppress the anti-inflammatory effects of glucocorticoids Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 12345, wild-type enzyme, mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
MIF
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
keto-(p-hydroxyphenyl)pyruvate 25°C, pH 6.0, mutant enzyme P1(A)M2 Homo sapiens
0.93
-
keto-(p-hydroxyphenyl)pyruvate 25°C, pH 6.0, mutant enzyme P1G Homo sapiens
2.7
-
keto-(p-hydroxyphenyl)pyruvate 25°C, pH 6.0, wild-type enzyme Homo sapiens
6.08
-
keto-(p-hydroxyphenyl)pyruvate 25°C, pH 6.0, mutant enzyme P1G Homo sapiens