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Literature summary for 5.3.1.9 extracted from

  • Hansen, T.; Schlichting, B.; Groetzinger, J.; Swan, M.K.; Davies, C.; Schoenheit, P.
    Mutagenesis of catalytically important residues of cupin type phosphoglucose isomerase from Archaeoglobus fulgidus (2005), FEBS J., 272, 6266-6275.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E93A Ni2+ content and apparent Ni2+ binding ability is reduced Archaeoglobus fulgidus
E93D catalytic efficiency is reduced by more than 100fold. Ni2+ content and apparent Ni2+ binding ability is reduced Archaeoglobus fulgidus
G79A mutant enzyme exhibits a lower thermostability, catalytic efficiency is reduced by more than 100fold Archaeoglobus fulgidus
G79L mutant enzyme exhibits a lower thermostability, catalytic efficiency is reduced by more than 100fold Archaeoglobus fulgidus
H136A catalytic efficiency remains about the same compared to wild-type activity Archaeoglobus fulgidus
H80A catalytic efficiency is reduced by more than 100fold. Ni2+ content and apparent Ni2+ binding ability is reduced Archaeoglobus fulgidus
H80D catalytic efficiency is reduced by more than 100fold. Ni2+ content and apparent Ni2+ binding ability is reduced Archaeoglobus fulgidus
H82A catalytic efficiency is reduced by more than 15fold, catalytic efficiency is reduced by more than 15fold. Ni2+ content and apparent Ni2+ binding ability is reduced Archaeoglobus fulgidus
T63A catalytic efficiency is reduced by more than 15fold Archaeoglobus fulgidus
Y160F catalytic efficiency is reduced by more than 15fold Archaeoglobus fulgidus
Y95F mutant enzyme exhibits a lower thermostability, catalytic efficiency is reduced by more than 100fold Archaeoglobus fulgidus
Y95K catalytic efficiency is reduced by more than 15fold Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y95K Archaeoglobus fulgidus
0.19
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H136A Archaeoglobus fulgidus
0.22
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme T63A Archaeoglobus fulgidus
0.25
-
D-fructose 6-phosphate pH 7.4, 70°C, wild-type enzyme Archaeoglobus fulgidus
0.36
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y95F Archaeoglobus fulgidus
0.39
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H80D Archaeoglobus fulgidus
0.5
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme G79A Archaeoglobus fulgidus
1.4
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y160F Archaeoglobus fulgidus
2
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme G79L Archaeoglobus fulgidus
2
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H82A Archaeoglobus fulgidus
2.9
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme E93D Archaeoglobus fulgidus
20.3
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H80A Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28778
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate
-
Archaeoglobus fulgidus D-glucose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
AfcPGI
-
Archaeoglobus fulgidus
Phosphoglucose isomerase
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
77
-
Tm-value of mutant enzyme G79L Archaeoglobus fulgidus
87
-
Tm-value of mutant enzyme G79A Archaeoglobus fulgidus
97
-
Tm-value of mutant enzyme H136A Archaeoglobus fulgidus
98
-
Tm-value of wild-type enzyme and mutant enzymes T63A, H80A, H80D, H82A, E93A, E93D, Y95K and Y160F Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme E93D Archaeoglobus fulgidus
0.07
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme G79L Archaeoglobus fulgidus
0.22
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y95F Archaeoglobus fulgidus
0.32
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme G79A Archaeoglobus fulgidus
0.42
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H80D Archaeoglobus fulgidus
0.43
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H80A Archaeoglobus fulgidus
0.68
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y95K Archaeoglobus fulgidus
1.9
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme T63A Archaeoglobus fulgidus
8
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H82A Archaeoglobus fulgidus
11.6
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y160F Archaeoglobus fulgidus
30.8
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H136A Archaeoglobus fulgidus
32.4
-
D-fructose 6-phosphate pH 7.4, 70°C, wild-type enzyme Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Archaeoglobus fulgidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme E93D Archaeoglobus fulgidus
0.02
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H80A Archaeoglobus fulgidus
0.035
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme G79L Archaeoglobus fulgidus
0.61
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y95F Archaeoglobus fulgidus
0.64
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme G79A Archaeoglobus fulgidus
1.1
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H80D Archaeoglobus fulgidus
3.8
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y95K Archaeoglobus fulgidus
4
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H82A Archaeoglobus fulgidus
8.3
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y160F Archaeoglobus fulgidus
8.6
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme T63A Archaeoglobus fulgidus
130
-
D-fructose 6-phosphate pH 7.4, 70°C, wild-type enzyme Archaeoglobus fulgidus
162
-
D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H136A Archaeoglobus fulgidus