Protein Variants | Comment | Organism |
---|---|---|
E93A | Ni2+ content and apparent Ni2+ binding ability is reduced | Archaeoglobus fulgidus |
E93D | catalytic efficiency is reduced by more than 100fold. Ni2+ content and apparent Ni2+ binding ability is reduced | Archaeoglobus fulgidus |
G79A | mutant enzyme exhibits a lower thermostability, catalytic efficiency is reduced by more than 100fold | Archaeoglobus fulgidus |
G79L | mutant enzyme exhibits a lower thermostability, catalytic efficiency is reduced by more than 100fold | Archaeoglobus fulgidus |
H136A | catalytic efficiency remains about the same compared to wild-type activity | Archaeoglobus fulgidus |
H80A | catalytic efficiency is reduced by more than 100fold. Ni2+ content and apparent Ni2+ binding ability is reduced | Archaeoglobus fulgidus |
H80D | catalytic efficiency is reduced by more than 100fold. Ni2+ content and apparent Ni2+ binding ability is reduced | Archaeoglobus fulgidus |
H82A | catalytic efficiency is reduced by more than 15fold, catalytic efficiency is reduced by more than 15fold. Ni2+ content and apparent Ni2+ binding ability is reduced | Archaeoglobus fulgidus |
T63A | catalytic efficiency is reduced by more than 15fold | Archaeoglobus fulgidus |
Y160F | catalytic efficiency is reduced by more than 15fold | Archaeoglobus fulgidus |
Y95F | mutant enzyme exhibits a lower thermostability, catalytic efficiency is reduced by more than 100fold | Archaeoglobus fulgidus |
Y95K | catalytic efficiency is reduced by more than 15fold | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y95K | Archaeoglobus fulgidus | |
0.19 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H136A | Archaeoglobus fulgidus | |
0.22 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme T63A | Archaeoglobus fulgidus | |
0.25 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, wild-type enzyme | Archaeoglobus fulgidus | |
0.36 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y95F | Archaeoglobus fulgidus | |
0.39 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H80D | Archaeoglobus fulgidus | |
0.5 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme G79A | Archaeoglobus fulgidus | |
1.4 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y160F | Archaeoglobus fulgidus | |
2 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme G79L | Archaeoglobus fulgidus | |
2 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H82A | Archaeoglobus fulgidus | |
2.9 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme E93D | Archaeoglobus fulgidus | |
20.3 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H80A | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28778 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate | - |
Archaeoglobus fulgidus | D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AfcPGI | - |
Archaeoglobus fulgidus |
Phosphoglucose isomerase | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
77 | - |
Tm-value of mutant enzyme G79L | Archaeoglobus fulgidus |
87 | - |
Tm-value of mutant enzyme G79A | Archaeoglobus fulgidus |
97 | - |
Tm-value of mutant enzyme H136A | Archaeoglobus fulgidus |
98 | - |
Tm-value of wild-type enzyme and mutant enzymes T63A, H80A, H80D, H82A, E93A, E93D, Y95K and Y160F | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme E93D | Archaeoglobus fulgidus | |
0.07 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme G79L | Archaeoglobus fulgidus | |
0.22 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y95F | Archaeoglobus fulgidus | |
0.32 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme G79A | Archaeoglobus fulgidus | |
0.42 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H80D | Archaeoglobus fulgidus | |
0.43 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H80A | Archaeoglobus fulgidus | |
0.68 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y95K | Archaeoglobus fulgidus | |
1.9 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme T63A | Archaeoglobus fulgidus | |
8 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H82A | Archaeoglobus fulgidus | |
11.6 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y160F | Archaeoglobus fulgidus | |
30.8 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H136A | Archaeoglobus fulgidus | |
32.4 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, wild-type enzyme | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Archaeoglobus fulgidus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme E93D | Archaeoglobus fulgidus | |
0.02 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H80A | Archaeoglobus fulgidus | |
0.035 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme G79L | Archaeoglobus fulgidus | |
0.61 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y95F | Archaeoglobus fulgidus | |
0.64 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme G79A | Archaeoglobus fulgidus | |
1.1 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H80D | Archaeoglobus fulgidus | |
3.8 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y95K | Archaeoglobus fulgidus | |
4 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H82A | Archaeoglobus fulgidus | |
8.3 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme Y160F | Archaeoglobus fulgidus | |
8.6 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme T63A | Archaeoglobus fulgidus | |
130 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, wild-type enzyme | Archaeoglobus fulgidus | |
162 | - |
D-fructose 6-phosphate | pH 7.4, 70°C, mutant enzyme H136A | Archaeoglobus fulgidus |