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Literature summary for 5.3.1.9 extracted from

  • Haller, J.F.; Smith, C.; Liu, D.; Zheng, H.; Tornheim, K.; Han, G.S.; Carman, G.M.; Zoeller, R.A.
    Isolation of novel animal cell lines defective in glycerolipid biosynthesis reveals mutations in glucose-6-phosphate isomerase (2010), J. Biol. Chem., 285, 866-877.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
G189E GroD1 enzyme mutant cells show 87% reduced enzyme activity and defects in glycerolipid biosynthesis, overview. The phenotype comprises profound reduction in the synthesis of phosphatidylcholine, phosphatidylethanolamine, and triglycerides but high levels of phosphatidic acid and normal levels of phosphatidylinositol synthesis, accompanied by a reduction in phosphatidate phosphatase 1 activity. Expression of wild-type hamster GPI restores GPI activity, glycerolipid biosynthesis, and PAP1 activity in GroD1 Cricetulus griseus
additional information independently isolated GPI-deficient mutants displayed similar phenotypes like G189E with respect to PAP1 activity and glycerolipid biosynthesis Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Cricetulus griseus
0.11
-
 D-glucose 6-phosphate ZR-82 cells, 22°C Cricetulus griseus
2.58
-
 D-glucose 6-phosphate mutant GroD1 cells, 22°C Cricetulus griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate Cricetulus griseus
-
 D-fructose 6-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
CHO-K1 cell
-
 Cricetulus griseus
-
ovary
-
 Cricetulus griseus
-
ZR-82 cell peroxisome/plasmalogen-deficient variant of the CHO-K1 cell line Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
 Cricetulus griseus D-fructose 6-phosphate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Cricetulus griseus

General Information

General Information Comment Organism
malfunction neuromuscular symptoms are associated with inherited GPI deficiency Cricetulus griseus