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Literature summary for 5.3.1.9 extracted from
- Crystallization and preliminary X-ray crystallographic study of phosphoglucose isomerase from Plasmodium falciparum (2010), Acta Crystallogr. Sect. F, 66, 333-336.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Plasmodium falciparum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing 0.002 ml of both protein solution and reservoir solution, the latter containing 38% v/v PEG 400 and 0.2 M calcium acetate in 0.1 M sodium cacodylate-HCl, pH 6.5, equilibration over 0.5 ml of reservoir solution, 1 week, X-ray diffraction structure determination and analysis at 1.5 A resolution | Plasmodium falciparum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | Plasmodium falciparum | - |
D-fructose 6-phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to homogeneity | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | - |
Plasmodium falciparum | D-fructose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glucose 6-phosphate isomerase | - |
Plasmodium falciparum |
| GPI | - |
Plasmodium falciparum |
| PGI | - |
Plasmodium falciparum |
| PHI | - |
Plasmodium falciparum |
| Phosphoglucose isomerase | - |
Plasmodium falciparum |
| Phosphohexose isomerase | - |
Plasmodium falciparum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | PGI is a key enzyme in glycolysis and glycogenesis catalyzing the second step of glycolysis | Plasmodium falciparum |
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