Crystallization (Comment) | Organism |
---|---|
in presence of bound zinc, substrate D-fructose 6-phosphate and several competitive inhibitors | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phospho-D-arabinonate | competitive | Pyrococcus furiosus | |
5-phospho-D-arabinonohydroxamate | competitive, stable analogue of putative cis-endiol intermediate | Pyrococcus furiosus | |
EDTA | 100fold excess, complete loss of activity within 10 min. 93% of activity may be recovered by additon of 1000fold excess of Zn2+ | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
D-fructose 6-phosphate | 50°C | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | P83194 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate | cis-endiol intermediate based mechanism with Glu97 acting as the catalytic base responsible for isomerization | Pyrococcus furiosus |
Renatured (Comment) | Organism |
---|---|
enzyme inactivated by 100fold excess of EDTA can be reactivated by 1000fold excess of of Zn2+ | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate | - |
Pyrococcus furiosus | D-glucose 6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.4 | - |
D-fructose 6-phosphate | 50°C | Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
5-phospho-D-arabinonohydroxamate | 50°C | Pyrococcus furiosus | |
0.27 | - |
5-phospho-D-arabinonate | 50°C | Pyrococcus furiosus |