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Literature summary for 5.3.1.8 extracted from

  • Sigdel, S.; Singh, R.; Kim, T.S.; Li, J.; Kim, S.Y.; Kim, I.W.; Jung, W.S.; Pan, C.H.; Kang, Y.C.; Lee, J.K.
    Characterization of a mannose-6-phosphate isomerase from Bacillus amyloliquefaciens and its application in fructose-6-phosphate production (2015), PLoS ONE, 10, e0131585 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus amyloliquefaciens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, complete loss of activity Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.62
-
 D-mannose 6-phosphate pH 7.5, 70°C Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ most effective activator of EDTA-inactivated enzyme, 1.7fold activation at 0.5 mM compared to initial activity Bacillus amyloliquefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-
Bacillus amyloliquefaciens DSM 7
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
81
-
 substrate D-mannose 6-phosphate, pH 7.5, 70°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Mannose 6-phosphate
-
 Bacillus amyloliquefaciens D-Fructose 6-phosphate
-
 ?
D-Mannose 6-phosphate
-
 Bacillus amyloliquefaciens DSM 7 D-Fructose 6-phosphate
-
 ?
D-ribose poor substrate Bacillus amyloliquefaciens D-ribulose
-
 ?
D-ribose poor substrate Bacillus amyloliquefaciens DSM 7 D-ribulose
-
 ?
L-ribulose poor substrate Bacillus amyloliquefaciens L-ribose
-
 ?
L-ribulose poor substrate Bacillus amyloliquefaciens DSM 7 L-ribose
-
 ?
L-talose poor substrate Bacillus amyloliquefaciens L-tagatose
-
 ?
L-talose poor substrate Bacillus amyloliquefaciens DSM 7 L-tagatose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35490, calculated from sequence, 1 * 35000, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

Synonyms Comment Organism
M6PI
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 presence of Co2+, half-life 15 h Bacillus amyloliquefaciens
50
-
 presence of Co2+, half-life 3.5 h Bacillus amyloliquefaciens
60
-
 presence of Co2+, half-life 10 min Bacillus amyloliquefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
106000
-
 D-mannose 6-phosphate pH 7.5, 70°C Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
4
-
 83% of maximum activity Bacillus amyloliquefaciens
9
-
 94% of maximum activity Bacillus amyloliquefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13900
-
 D-mannose 6-phosphate pH 7.5, 70°C Bacillus amyloliquefaciens