Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Geobacillus thermodenitrificans |
Protein Variants | Comment | Organism |
---|---|---|
L129F | the activity of the mutant is 204% of the activity of the wild type enzyme | Geobacillus thermodenitrificans |
L129W | the activity of the mutant is 10% of the activity of the wild type enzyme | Geobacillus thermodenitrificans |
L129Y | the activity of the mutant is 94% of the activity of the wild type enzyme | Geobacillus thermodenitrificans |
N90A/L129F | the activity of the mutant is about 6fold higher than the activity of the wild type enzyme | Geobacillus thermodenitrificans |
W17Q/N90A/L129F | the specific activity and catalytic efficiency for L-ribulose isomerization of this variant are 3.1 and 7.1fold higher, respectively, than those of the wild type enzyme at pH 7.0 and 70°C in the presence of 1mM Co2+ | Geobacillus thermodenitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | 9 | D-mannose 6-phosphate | mutant enzyme N90A/L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
100 | - |
D-mannose 6-phosphate | mutant enzyme W17Q/N90A/L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
149 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
243 | - |
D-mannose 6-phosphate | mutant enzyme L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | strong stimulation of activity | Geobacillus thermodenitrificans | |
Cu2+ | significantly stimulated by Cu2+ | Geobacillus thermodenitrificans | |
Mn2+ | significantly stimulated by Mn2+ | Geobacillus thermodenitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | Geobacillus thermodenitrificans | - |
D-Fructose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A4ITT1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | - |
Geobacillus thermodenitrificans | D-Fructose 6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23550 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
55120 | - |
D-mannose 6-phosphate | mutant enzyme L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
85720 | - |
D-mannose 6-phosphate | mutant enzyme N90A/L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
112100 | - |
D-mannose 6-phosphate | mutant enzyme W17Q/N90A/L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
158 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
227 | - |
D-mannose 6-phosphate | mutant enzyme L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
965 | - |
D-mannose 6-phosphate | mutant enzyme N90A/L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans | |
1120 | - |
D-mannose 6-phosphate | mutant enzyme W17Q/N90A/L129F, at pH 7.0 and 70°C | Geobacillus thermodenitrificans |