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Literature summary for 5.3.1.8 extracted from

  • Roux, C.; Bhatt, F.; Foret, J.; de Courcy, B.; Gresh, N.; Piquemal, J.P.; Jeffery, C.J.; Salmon, L.
    The reaction mechanism of type I phosphomannose isomerases: new information from inhibition and polarizable molecular mechanics studies (2011), Proteins, 79, 203-220.
    View publication on PubMed
Search for more literature for 5.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli GI724 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-phospho-D-arabinonhydrazide
-
 Escherichia coli
5-phospho-D-arabinonhydrazide in 50 mM HEPES buffer, pH 7.1, at 25°C Homo sapiens
5-phospho-D-arabinonohydroxamic acid nanomolar inhibitor Escherichia coli
5-phospho-D-arabinonohydroxamic acid in 50 mM HEPES buffer, pH 7.1, at 25°C; nanomolar inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00043
-
 beta-D-mannose 6-phosphate in 50 mM HEPES buffer, pH 7.1, at 25°C Homo sapiens
0.33
-
 beta-D-mannose 6-phosphate in 50 mM HEPES buffer, pH 7.1, at 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-dependent metalloenzyme Escherichia coli
Zn2+ zinc-dependent metalloenzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Ni-chelating column chromatography and Q-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-mannose 6-phosphate
-
 Escherichia coli beta-D-fructose 6-phosphate
-
 r
beta-D-mannose 6-phosphate
-
 Homo sapiens beta-D-fructose 6-phosphate
-
 r

Synonyms

Synonyms Comment Organism
type I phosphomannose isomerase
-
 Escherichia coli
type I phosphomannose isomerase
-
 Homo sapiens
type I PMI
-
 Escherichia coli
type I PMI
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 beta-D-mannose 6-phosphate in 50 mM HEPES buffer, pH 7.1, at 25°C Homo sapiens
23
-
 beta-D-mannose 6-phosphate in 50 mM HEPES buffer, pH 7.1, at 25°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000041
-
 5-phospho-D-arabinonohydroxamic acid in 50 mM HEPES buffer, pH 7.1, at 25°C Homo sapiens
0.00008
-
 5-phospho-D-arabinonohydroxamic acid in 50 mM HEPES buffer, pH 7.1, at 25°C Escherichia coli
0.0006
-
 5-phospho-D-arabinonhydrazide in 50 mM HEPES buffer, pH 7.1, at 25°C Homo sapiens
0.002
-
 5-phospho-D-arabinonhydrazide in 50 mM HEPES buffer, pH 7.1, at 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
70
-
 beta-D-mannose 6-phosphate in 50 mM HEPES buffer, pH 7.1, at 25°C Escherichia coli
840
-
 beta-D-mannose 6-phosphate in 50 mM HEPES buffer, pH 7.1, at 25°C Homo sapiens