Literature summary for 5.3.1.8 extracted from

Sousa, S.A.; Moreira, L.M.; Wopperer, J.; Eberl, L.; Sa-Correia, I.; Leitao, J.H.
The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities (2007), Biochem. Biophys. Res. Commun., 353, 200-206.

Search for more literature for 5.3.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
-	Burkholderia cepacia

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme-deficient mutant is not impaired in synthesis of exopolysaccharide. However, the viscosity of aqueous solutions prepared with the exopolysaccharide produced by the mutant is significantly reduced compared with wild-type biopolymer and the mutant forms biofilms with a size reduced by 6fold	Burkholderia cepacia

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	2 mM, complete inhibition	Burkholderia cepacia
additional information	not inhibitory: mannose 1-phosphate, GTP, GDP-mannose, or diphosphate	Burkholderia cepacia

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
9	-	D-mannose 6-phosphate	pH 7.6, presence of 5 mM Mg2+	Burkholderia cepacia

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	best activator, may be replaced by Mg2+ or Mn2+ for phosphomannose isomerase activity	Burkholderia cepacia
Mg2+	60% of activity with Ca2+ for phosphomannose isomerase activity	Burkholderia cepacia
Mn2+	65% of activity with Ca2+ for phosphomannose isomerase activity	Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	x * 55300, calculated, x * 55000, SDS-PAGE	Burkholderia cepacia
55300	-	x * 55300, calculated, x * 55000, SDS-PAGE	Burkholderia cepacia

Organism

Organism	UniProt	Comment	Textmining
Burkholderia cepacia	Q06XM8	type II enzyme, bifunctional protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Mannose 6-phosphate	-	Burkholderia cepacia	D-Fructose 6-phosphate	-	?
D-Mannose 6-phosphate	-	Burkholderia cepacia IST408	D-Fructose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 55300, calculated, x * 55000, SDS-PAGE	Burkholderia cepacia

Synonyms

Synonyms	Comment	Organism
becA	-	Burkholderia cepacia

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
19.4	-	D-mannose 6-phosphate	pH 7.6, presence of 5 mM Mg2+	Burkholderia cepacia

pI Value

Organism	Comment	pI Value Maximum	pI Value
Burkholderia cepacia	calculated	-	6

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Release 2023.1 (January 2023)