Literature summary for 5.3.1.8 extracted from

Hansen, T.; Wendorff, D.; Schonheit, P.
Bifunctional phosphoglucose/phosphomannose isomerases from the Archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily (2004), J. Biol. Chem., 279, 2262-2272.

Search for more literature for 5.3.1.8

Inhibitors

Inhibitors	Comment	Organism	Structure
6-phosphogluconate	-	Aeropyrum pernix
6-phosphogluconate	-	Thermoplasma acidophilum
erythrose 4-phosphate	-	Aeropyrum pernix
erythrose 4-phosphate	-	Thermoplasma acidophilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	D-fructose 6-phosphate	80°C, pH 7.4	Thermoplasma acidophilum
0.21	-	D-fructose 6-phosphate	50°C, pH 7.4	Aeropyrum pernix
0.25	-	D-mannose 6-phosphate	80°C, pH 7.4	Thermoplasma acidophilum
0.44	-	D-fructose 6-phosphate	80°C, pH 7.4	Aeropyrum pernix
0.72	-	D-glucose 6-phosphate	80°C, pH 7.4	Thermoplasma acidophilum
1.1	-	D-mannose 6-phosphate	80°C, pH 7.4	Aeropyrum pernix
3.5	-	D-glucose 6-phosphate	80°C, pH 7.4	Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	2 * 35000, SDS-PAGE, 2 * 35150, calculated	Thermoplasma acidophilum
35150	-	2 * 35000, SDS-PAGE, 2 * 35150, calculated	Thermoplasma acidophilum
36000	-	2 * 36000, SDS-PAGE, 2 * 36100, calculated	Aeropyrum pernix
36100	-	2 * 36000, SDS-PAGE, 2 * 36100, calculated	Aeropyrum pernix
45000	-	gel filtration	Aeropyrum pernix
48000	-	gel filtration	Thermoplasma acidophilum
67000	-	analytical ultracentrifugation	Thermoplasma acidophilum

Organism

Organism	UniProt	Comment	Textmining
Aeropyrum pernix	Q9YE01	biunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.9 and 5.3.1.8, resp.	-
Thermoplasma acidophilum	Q9HIC2	biunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.9 and 5.3.1.8, resp.	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 6-phosphate	-	Aeropyrum pernix	D-glucose 6-phosphate	-	r
D-fructose 6-phosphate	-	Thermoplasma acidophilum	D-glucose 6-phosphate	-	r
D-fructose 6-phosphate	-	Aeropyrum pernix	D-mannose 6-phosphate	-	r
D-fructose 6-phosphate	-	Thermoplasma acidophilum	D-mannose 6-phosphate	-	r
D-glucose 6-phosphate	-	Aeropyrum pernix	D-fructose 6-phosphate	-	r
D-glucose 6-phosphate	-	Thermoplasma acidophilum	D-fructose 6-phosphate	-	r
D-Mannose 6-phosphate	-	Aeropyrum pernix	D-Fructose 6-phosphate	-	r
D-Mannose 6-phosphate	-	Thermoplasma acidophilum	D-Fructose 6-phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 35000, SDS-PAGE, 2 * 35150, calculated	Thermoplasma acidophilum
dimer	2 * 36000, SDS-PAGE, 2 * 36100, calculated	Aeropyrum pernix

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.035	-	erythrose 4-phosphate	80°C, pH 7.4	Aeropyrum pernix
0.164	-	erythrose 4-phosphate	80°C, pH 7.4	Thermoplasma acidophilum
2	10	6-phosphogluconate	80°C, pH 7.4	Thermoplasma acidophilum
58	-	6-phosphogluconate	80°C, pH 7.4	Aeropyrum pernix

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Release 2023.1 (January 2023)