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Literature summary for 5.3.1.8 extracted from
- Phosphomannose isomerase of Xanthomonas campestris: a zinc activated enzyme (1997), Mol. Cell. Biochem., 177, 183-191.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldicarbonate | - |
Xanthomonas campestris |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
mannose 6-phosphate | - |
Xanthomonas campestris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Xanthomonas campestris | |
| Co2+ | activates | Xanthomonas campestris | |
| Mn2+ | activates | Xanthomonas campestris | |
| additional information | activated by bivalent cations in decreasing order: Co2+, Zn2+, Mn2+, Ni2+, Ca2+ | Xanthomonas campestris | |
| Ni2+ | - |
Xanthomonas campestris | |
| Zn2+ | activates | Xanthomonas campestris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Xanthomonas campestris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas campestris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Xanthomonas campestris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33.5 | - |
- |
Xanthomonas campestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Mannose 6-phosphate | - |
Xanthomonas campestris | D-Fructose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Xanthomonas campestris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
half-life in absence of Zn2+: 110 min, half-life in presence of 0.0002 mM ZnCl2: 270 min | Xanthomonas campestris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Xanthomonas campestris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | 4°C, stable | Xanthomonas campestris |
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Release 2023.1 (January 2023)
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