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Literature summary for 5.3.1.8 extracted from
- Purification, cDNA cloning and heterologous expression of human phosphomannose isomerase (1994), Eur. J. Biochem., 219, 415-423.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA under the control of the GAL1 promoter, expression in Saccharomyces cerevisiae and in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
mannose 6-phosphate | recombinant enzyme | Homo sapiens |  |
| 0.25 | - |
mannose 6-phosphate | wild-type enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46520 | - |
calculation from nucleotide sequence encoded by cDNA | Homo sapiens |
| 46530 | - |
recombinant enzyme, electrospray mass spectroscopy | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | critical role in the supply of the D-mannose derivatives required for many glycosylation reactions | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Mannose 6-phosphate | - |
Homo sapiens | D-Fructose 6-phosphate | - |
? | |
| additional information | critical role in the supply of the D-mannose derivatives required for many glycosylation reactions | Homo sapiens | ? | - |
? | |
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